| Detection of free radical-induced DNA damage with bromodeoxyuridine/Hoechst flow cytometry: implications for Bloom's syndrome. | |
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MedLine Citation:
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PMID: 1692968 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The clinical radiosensitizer bromodeoxyuridine (BrdU) was shown to enhance oxygen free radical-mediated growth inhibition. Cells from Bloom's syndrome, a rare autosomal recessive disorder characterized by pre- and post-natal growth deficits, telangiectatic erythema, recurrent respiratory infections and a high incidence of cancer, exhibit in culture a hypersensitivity to BrdU. We analysed disturbed cell kinetics of Bloom's syndrome fibroblasts and permanent B-cell lines with a novel cell kinetic method: BrdU/Hoechst flow cytometry. Fibroblasts show a pattern similar to that of normal cells exposed to a breakdown product of lipid peroxides, whereas B-cells exhibit the cell kinetic disturbance provoked by elevated oxygen concentrations in normal cells. In both cell types the cell kinetic pattern was dependent upon the BrdU concentration in the culture medium. These data suggest an elevated endogenous generation of oxygen free radicals in Bloom's syndrome cells, which may relate to the elevated incidence of malignancies in these patients. |
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Authors:
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M Poot; H W Rüdiger; H Hoehn |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Mutation research Volume: 238 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 1990 May |
Date Detail:
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Created Date: 1990-06-25 Completed Date: 1990-06-25 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 203-7 Citation Subset: IM |
Affiliation:
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Department of Human Genetics, University of Würzburg, F.R.G. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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B-Lymphocytes
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physiology Bisbenzimidazole Bloom Syndrome / genetics* Bromodeoxyuridine Cell Cycle Cell Transformation, Viral DNA / analysis* DNA Damage* Fibroblasts / physiology Flow Cytometry Free Radicals Herpesvirus 4, Human Humans Oxygen / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Free Radicals; 23491-44-3/Bisbenzimidazole; 59-14-3/Bromodeoxyuridine; 7782-44-7/Oxygen; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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