Document Detail

Detection of fetal Rhesus D gene in whole blood of women booking for routine antenatal care.
MedLine Citation:
PMID:  12694966     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine if the RhD status of the fetus can be detected in an unselected group of RhD-negative women with ongoing pregnancies booking for routine antenatal care. MATERIAL AND METHODS: We obtained 2.5 ml of whole blood from 202 unselected women with a normal ongoing pregnancy who booked for antenatal care before 20 weeks' gestation. DNA was extracted and real time quantitative PCR performed. Primers and a specific probe were targeted at the Rhesus D gene. RESULTS: Of 194 women, 31 were RhD-negative with no evidence of prior isoimmunisation. They delivered 17 RhD-positive and 14 RhD-negative babies. Of the 17 RhD-negative women carrying a RhD-positive fetus, the RhD gene was detected in the maternal blood in 14 (82%). There were no false positives. The quantity of RhD gene detected in the maternal blood of RhD-negative mothers ranged from 2 x 10(2) to 4 x 10(6) copies/ml of whole blood. CONCLUSION: Using real time quantitative PCR assays to amplify free fetal DNA in the maternal circulation, identification of RhD-positive fetuses in RhD-negative mothers is feasible when they book for antenatal care. Before such RhD genotyping can be introduced into clinical practice, however, further studies are required to show that false negative results can be eliminated and to show that this diagnostic test is reliable outside a research setting.
Michael J Turner; Cara M Martin; John J O'Leary
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of obstetrics, gynecology, and reproductive biology     Volume:  108     ISSN:  0301-2115     ISO Abbreviation:  Eur. J. Obstet. Gynecol. Reprod. Biol.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-15     Completed Date:  2004-01-16     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0375672     Medline TA:  Eur J Obstet Gynecol Reprod Biol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  29-32     Citation Subset:  IM    
Department of Obstetrics and Gynaecology, Coombe Women's Hospital, Dolphin's Barn, Dublin 8, Ireland.
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MeSH Terms
DNA / blood*
Gestational Age
Polymerase Chain Reaction
Prenatal Care*
Rh Isoimmunization / blood
Rh-Hr Blood-Group System / genetics*
Reg. No./Substance:
0/Rh-Hr Blood-Group System; 9007-49-2/DNA
Comment In:
Eur J Obstet Gynecol Reprod Biol. 2003 Sep 10;110(1):117; author reply 118   [PMID:  12932887 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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