Document Detail


Detection of factor IX inhibitors by immunoradiometric assay.
MedLine Citation:
PMID:  6414224     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An immunoradiometric assay (IRMA) for the determination of factor IX inhibitors in haemophilia B was developed. The assay was based on competitive binding between radiolabelled anti-IX and inhibitors in the test material of immobilized IX:C. 5 haemophiliacs with known inhibitors were investigated with the new method and with a conventional neutralization test. An inhibitor titre as low as 5 X 10(-4) U/ml could be measured by IRMA, showing it to be about 500 times as sensitive as the conventional neutralization assay used, and in 2 cases the inhibitors could only be detected by IRMA. The new method is thus useful for the investigation of patients with low inhibitor titres, or when inhibitors with divergent properties are suspected.
Authors:
R Ljung; L Holmberg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta haematologica     Volume:  70     ISSN:  0001-5792     ISO Abbreviation:  Acta Haematol.     Publication Date:  1983  
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0141053     Medline TA:  Acta Haematol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  307-11     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antibodies / analysis*,  isolation & purification
Binding Sites, Antibody
Binding, Competitive
Blood Coagulation*
Factor IX / antagonists & inhibitors*,  immunology
Hemophilia B / blood*
Neutralization Tests
Radioimmunoassay
Chemical
Reg. No./Substance:
0/Antibodies; 0/Binding Sites, Antibody; 9001-28-9/Factor IX

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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