Document Detail

Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women.
MedLine Citation:
PMID:  23018230     Owner:  NLM     Status:  In-Data-Review    
The aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. This cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. Probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. Subgingival plaque samples were collected and the FISH technique identified the presence and numbers of Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobacter rectus, Porphyromonas gingivalis, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia and Prevotella nigrescens. The Mann-Whitney U-test was applied to compare the data between the two groups. The mean age, ethnicity, marital status, education, and economic level in both groups were similar. The clinical parameters showed no significant differences between pregnant and non-pregnant women. The numbers of subgingival periodontopathogens were not found to be significantly different between groups, despite the higher mean counts of P. intermedia in pregnant women. Colonization patterns of the different bacteria most commonly associated with periodontal disease were not different in the subgingival plaque of pregnant and non-pregnant women.
Fernanda Campos Machado; Dionéia Evangelista Cesar; Amanda Vervloet Dutra Agostinho Assis; Cláudio Galuppo Diniz; Rosangela Almeida Ribeiro
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Brazilian oral research     Volume:  26     ISSN:  1807-3107     ISO Abbreviation:  Braz Oral Res     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101307187     Medline TA:  Braz Oral Res     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  443-9     Citation Subset:  D; IM    
School of Medicine, Univ Federal de Juiz de Fora - UFJF, Juiz de Fora, MG, Brazil.
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