Document Detail


Detection of enterovirus capsid protein VP1 in myocardium from cases of myocarditis or dilated cardiomyopathy by immunohistochemistry: further evidence of enterovirus persistence in myocytes.
MedLine Citation:
PMID:  14634804     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The association of enteroviruses with myocardial disease has been investigated extensively by molecular biological techniques to detect viral RNA, but remains controversial. This retrospective study investigated the involvement of enterovirus in myocarditis or dilated cardiomyopathy (DCM) by detection of viral antigens in myocardial samples from a new patient series using an optimized immunohistochemical technique. Formalin-fixed, paraffin-embedded biopsy, autopsy or explanted myocardial tissue samples were obtained from 136 subjects. These comprised histologically proven cases of acute fatal myocarditis (n=10), DCM (n=89, including 10 patients with healing/borderline myocarditis) and a comparison group of samples from 37 unused donor hearts and cases with other conditions. A monoclonal antibody 5-D8/1 directed against a conserved, non-conformational epitope in capsid protein VP1 was employed for broad detection of different enterovirus serotypes. Investigations were performed blindly. Histological sections from 7 of 10 fatal myocarditis cases, 47 of 89 patients (52.8%) with DCM were positive for the viral capsid protein VP1 by immunohistochemical staining. Consecutive sections of positive samples were negative when the antibody was omitted or replaced with subclass- and concentration-matched normal mouse IgG. In contrast, only 3 of 37 samples (8.1%) in the comparison group were positive (Yates corrected chi(2)=19.99, P<0.001: odds ratio =12.68). VP1 staining was distributed in individual or grouped myofibers and localized in the cytoplasm of myocytes. In some cases, VP1 was detected in only a few myofibers within an entire section. These results provide further evidence of enterovirus involvement in a high proportion of DCM cases and demonstrate that VP1 is present in disease stages from acute myocarditis, healing myocarditis to end-stage DCM requiring cardiac transplantation, indicating translation of viral protein during persistent enterovirus infection.
Authors:
Hongyi Zhang; Yanwen Li; Dougal R McClean; Peter J Richardson; Richard Florio; Mary Sheppard; Karen Morrison; Najma Latif; Michael J Dunn; Leonard C Archard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-11-22
Journal Detail:
Title:  Medical microbiology and immunology     Volume:  193     ISSN:  0300-8584     ISO Abbreviation:  Med. Microbiol. Immunol.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-10     Completed Date:  2004-07-27     Revised Date:  2007-02-21    
Medline Journal Info:
Nlm Unique ID:  0314524     Medline TA:  Med Microbiol Immunol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  109-14     Citation Subset:  IM    
Affiliation:
Cell and Molecular Biology Section, Division of Biomedical Sciences, Faculty of Medicine, Imperial College, London, UK. hongyi.zhang@addenbrookes.nhs.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Cardiomyopathy, Dilated / virology*
DNA-Binding Proteins / analysis*
Enterovirus / metabolism*
Enterovirus Infections / virology
Heart / virology
Humans
Immunohistochemistry
Mice
Myocarditis / virology*
Myocardium / chemistry*
Plant Proteins
Trans-Activators
Transcription Factors / analysis*
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Plant Proteins; 0/Trans-Activators; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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