| Detection and downregulation of type I IGF receptor expression by antibody-conjugated quantum dots in breast cancer cells. | |
| | |
MedLine Citation:
|
PMID: 18418709 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The type I insulin-like growth factor (IGF) receptor (IGF1R) is a transmembrane tyrosine kinase involved in breast cancer proliferation, survival, and metastasis. Several monoclonal antibodies directed against the receptor are in clinical trials. In order to develop a methodology to detect and measure IGF1R levels in breast cancer cells, we covalently conjugated an IGF1R antibody, AVE-1642, with quantum dots (Qdots), which are nanocrystals that emit fluorescence upon excitation. AVE-1642 Qdots only bound to cells that express IGF1R, and measured IGF1R levels by fluorescence emission at 655 nm. After binding to the cell surface, AVE-1642 Qdots underwent receptor mediated endocytosis, localized to endosome, and later translocated into the nucleus. Treating MCF-7 cells with AVE-1642 Qdots, but not unconjugated Qdots alone, downregulated IGF1R levels and rendered cells refractory to IGF-I stimulation. Furthermore, cell proliferation was slightly inhibited by AVE-1642 Qdots, but not the unconjugated Qdots. Our data suggest that AVE-1642 Qdots can be used to detect IGF1R expression and measure changes in cell surface receptor levels. In addition, the inhibitory effect of AVE-1642 Qdots to cell proliferation implies that it may serve as a traceable therapeutic agent. |
| | |
Authors:
|
Hua Zhang; Deepali Sachdev; Chun Wang; Allison Hubel; Martine Gaillard-Kelly; Douglas Yee |
Related Documents
:
|
8785049 - A reciprocal cell-cell interaction mediated by nt-3 and neuregulins controls the early ... 19832839 - Recent insights into pdgf-induced gliomagenesis. 7711939 - Fibroblast growth factors and insulin growth factors combine to promote survival of rat... 1660309 - Selective expression of high molecular weight basic fibroblast growth factor confers a ... 15089039 - Signal transduction of the protective effect of insulin like growth factor-1 on adriamy... 11231069 - Misexpression of igf-i in the mouse lens expands the transitional zone and perturbs len... 11536999 - Induction of chromosome aberrations in mammalian cells after heavy ion exposure. 17297979 - Single-cell detection: test of microbial contamination using capillary electrophoresis. 8608009 - Starting the cell cycle: what's the point? |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-04-17 |
Journal Detail:
|
Title: Breast cancer research and treatment Volume: 114 ISSN: 1573-7217 ISO Abbreviation: Breast Cancer Res. Treat. Publication Date: 2009 Mar |
Date Detail:
|
Created Date: 2009-02-04 Completed Date: 2009-04-07 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 8111104 Medline TA: Breast Cancer Res Treat Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 277-85 Citation Subset: IM |
Affiliation:
|
University of Minnesota Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antibodies, Monoclonal / immunology* Blotting, Western Breast Neoplasms / metabolism*, pathology Cell Line Cell Membrane / metabolism* Cell Proliferation Down-Regulation Female Fibroblasts / metabolism, pathology Flow Cytometry Homozygote Humans Insulin-Like Growth Factor I / pharmacology Mice Mice, Knockout Quantum Dots* Receptor, IGF Type 1 / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
P30 CA077598/CA/NCI NIH HHS; R01 CA74285/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 67763-96-6/Insulin-Like Growth Factor I; EC 2.7.10.1/Receptor, IGF Type 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Sexual self schema as a moderator of sexual and psychological outcomes for gynecologic cancer surviv...
Next Document: Micro-flow visualization of red blood cell-enhanced platelet concentration at sudden expansion.