Document Detail


Detection of disordered regions in globular proteins using ¹³C-detected NMR.
MedLine Citation:
PMID:  23047544     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Characterization of disordered regions in globular proteins constitutes a significant challenge. Here, we report an approach based on ¹³C-detected nuclear magnetic resonance experiments for the identification and assignment of disordered regions in large proteins. Using this method, we demonstrate that disordered fragments can be accurately identified in two homologs of menin, a globular protein with a molecular weight over 50 kDa. Our work provides an efficient way to characterize disordered fragments in globular proteins for structural biology applications.
Authors:
Felicia L V Gray; Marcelo J Murai; Jolanta Grembecka; Tomasz Cierpicki
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Protein science : a publication of the Protein Society     Volume:  21     ISSN:  1469-896X     ISO Abbreviation:  Protein Sci.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-27     Completed Date:  2013-04-29     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  9211750     Medline TA:  Protein Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1954-60     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 The Protein Society.
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MeSH Terms
Descriptor/Qualifier:
Animals
Humans
Models, Molecular
Nuclear Magnetic Resonance, Biomolecular / methods*
Protein Conformation
Proto-Oncogene Proteins / chemistry*
Sea Anemones / chemistry*
Grant Support
ID/Acronym/Agency:
1R01CA160467/CA/NCI NIH HHS; R01 CA160467/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/MEN1 protein, human; 0/Proto-Oncogene Proteins
Comments/Corrections

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