Document Detail

Detection of disease-specific augmentation of abnormal immunoglobulin G in sera of patients with rheumatoid arthritis.
MedLine Citation:
PMID:  10052597     Owner:  NLM     Status:  MEDLINE    
Galactose-free immunoglobulin G (IgG), which is known to be higher in the sera of patients with rheumatoid arthritis, was prepared from IgG of healthy volunteers using enzymes. Its reactivity to lectins was analyzed. The galactose-free IgG showed no reactivity to Ricinus communis agglutinin 120 but displayed greater reactivity to concanavalin A and Lens culinaris lectin than did intact human IgG. Then, IgG in serum samples was bound to protein A immobilized on a nitrocellulose membrane, and its reactivity to biotinylated concanavalin A was measured with streptavidin-conjugated horseradish peroxidase. When the reactivity to concanavalin A of IgG in sera from healthy individuals and patients with rheumatoid arthritis (RA), osteoarthritis, systemic lupus erythematosus, or hepatic disease was compared, higher levels were shown in patients with RA, notably in 60% of the seronegative patients and 80% of the early phase patients. Therefore, it was suggested that augmentation of the abnormal IgG in sera was highly specific to patients with RA and that this novel serum test could be very useful for an accurate diagnosis of this disease.
W Tang; A Matsumoto; K Shikata; F Takeuchi; T Konishi; M Nakata; T Mizuochi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glycoconjugate journal     Volume:  15     ISSN:  0282-0080     ISO Abbreviation:  Glycoconj. J.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1999-05-04     Completed Date:  1999-05-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  929-34     Citation Subset:  IM    
Department of Applied Chemistry, Tokai University, Hiratsuka, Kanagawa, Japan.
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MeSH Terms
Arthritis, Rheumatoid / blood,  immunology*
Carbohydrate Sequence
Concanavalin A / metabolism
Galactose / immunology
Immunoglobulin G / blood*
Lectins / metabolism
Molecular Sequence Data
Oligosaccharides / chemistry
Plant Lectins*
Protein Binding
Staphylococcal Protein A / metabolism
Reg. No./Substance:
0/Immunoglobulin G; 0/Lectins; 0/Oligosaccharides; 0/Plant Lectins; 0/Ricinus communis agglutinin-1; 0/Staphylococcal Protein A; 0/lentil lectin; 11028-71-0/Concanavalin A; 26566-61-0/Galactose

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