Document Detail


Detection of dimethylarginines in protein hydrolysates by matrix-assisted laser desorption/ionization mass spectrometry.
MedLine Citation:
PMID:  16403427     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report a method to detect the presence of dimethylarginines on proteins. Peptides with dimethylarginines were hydrolyzed in acid. The hydrolysates were subjected to matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometric analysis using a mixture of alpha-cyano-4-hydroxycinnamic acid and nitrocellulose as matrix. Both asymmetric omega-N(G),N(G)-dimethylarginine and symmetric omega-N(G),N(G')-dimethylarginine give a clear signal at m/z 203. Recombinant Sbp1p modified by Hmt1p in vivo were isolated by affinity chromatography followed by electrophoresis on a polyacrylamide gel and subjected to acid hydrolysis. MALDI-TOF analysis of the acid hydrolysates confirmed the presence of dimethylarginines. The detection limit of the method is estimated at approximately 1pmol of protein.
Authors:
Cheng-Hsilin Hsieh; Ming F Tam
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-12-20
Journal Detail:
Title:  Analytical biochemistry     Volume:  350     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-20     Completed Date:  2006-04-27     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  151-5     Citation Subset:  IM    
Affiliation:
Institute of Molecular Biology, Academia Sinica, NanKang, Taipei, Taiwan, ROC.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arginine / analogs & derivatives*,  analysis,  chemistry
Blood Proteins / chemistry
Collodion / chemistry
Coumaric Acids / chemistry
Fish Proteins / chemistry
Protein Hydrolysates / chemistry
Protein-Arginine N-Methyltransferases / metabolism
Repressor Proteins / metabolism
Saccharomyces cerevisiae Proteins / metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Chemical
Reg. No./Substance:
0/Blood Proteins; 0/Coumaric Acids; 0/Fish Proteins; 0/Protein Hydrolysates; 0/Repressor Proteins; 0/SBP1 protein, Parablax neblifer; 0/Saccharomyces cerevisiae Proteins; 0/dimethylarginine; 28166-41-8/alpha-cyano-4-hydroxycinnamate; 30315-93-6/N,N-dimethylarginine; 74-79-3/Arginine; 9004-70-0/Collodion; EC 2.1.1.-/HMT1 protein, S cerevisiae; EC 2.1.1.-/Protein-Arginine N-Methyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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