| Detection of dimethylarginines in protein hydrolysates by matrix-assisted laser desorption/ionization mass spectrometry. | |
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MedLine Citation:
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PMID: 16403427 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report a method to detect the presence of dimethylarginines on proteins. Peptides with dimethylarginines were hydrolyzed in acid. The hydrolysates were subjected to matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometric analysis using a mixture of alpha-cyano-4-hydroxycinnamic acid and nitrocellulose as matrix. Both asymmetric omega-N(G),N(G)-dimethylarginine and symmetric omega-N(G),N(G')-dimethylarginine give a clear signal at m/z 203. Recombinant Sbp1p modified by Hmt1p in vivo were isolated by affinity chromatography followed by electrophoresis on a polyacrylamide gel and subjected to acid hydrolysis. MALDI-TOF analysis of the acid hydrolysates confirmed the presence of dimethylarginines. The detection limit of the method is estimated at approximately 1pmol of protein. |
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Authors:
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Cheng-Hsilin Hsieh; Ming F Tam |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-12-20 |
Journal Detail:
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Title: Analytical biochemistry Volume: 350 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2006-02-20 Completed Date: 2006-04-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 151-5 Citation Subset: IM |
Affiliation:
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Institute of Molecular Biology, Academia Sinica, NanKang, Taipei, Taiwan, ROC. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arginine / analogs & derivatives*, analysis, chemistry Blood Proteins / chemistry Collodion / chemistry Coumaric Acids / chemistry Fish Proteins / chemistry Protein Hydrolysates / chemistry Protein-Arginine N-Methyltransferases / metabolism Repressor Proteins / metabolism Saccharomyces cerevisiae Proteins / metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* |
| Chemical | |
Reg. No./Substance:
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0/Blood Proteins; 0/Coumaric Acids; 0/Fish Proteins; 0/Protein Hydrolysates; 0/Repressor Proteins; 0/SBP1 protein, Parablax neblifer; 0/Saccharomyces cerevisiae Proteins; 0/dimethylarginine; 28166-41-8/alpha-cyano-4-hydroxycinnamate; 30315-93-6/N,N-dimethylarginine; 74-79-3/Arginine; 9004-70-0/Collodion; EC 2.1.1.-/HMT1 protein, S cerevisiae; EC 2.1.1.-/Protein-Arginine N-Methyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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