| Detection of dentin sialoprotein in rat periodontium. | |
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MedLine Citation:
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PMID: 15056114 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cloning and sequencing of the cDNA indicates that dentin sialophosphoprotein (DSPP) is a precursor of both dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). Dentin sialophosphoprotein must be proteolytically processed to form these two extracellular matrix (ECM) proteins. Numerous studies led us to conclude that DSP (and DSPP) are exclusively expressed by odontoblasts and preameloblasts. However, recent observations suggest a wider distribution. To test this hypothesis, we conducted systematic studies on rat first molar during root formation with immunohistochemical techniques using specific anti-DSP polyclonal and monoclonal antibodies. We also performed in situ hybridization, using high-stringency RNA probes to detect DSP transcripts. Immunohistochemical studies demonstrated that DSP is not only localized in odontoblasts, dentin ECM and preameloblasts, but also in alveolar bone, cellular cementum, osteocytes, cementocytes, and their matrices. The results of in situ hybridization were consistent with those from immunohistochemistry, showing the expression of DSP transcripts in osteoblasts of alveolar bone, fibroblasts in periodontal ligament and cementoblasts in cellular cementum. Together, these observations suggest that DSP is involved in formation of the periodontium as well as tooth structures. |
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Authors:
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Otto Baba; Chunlin Qin; Jan C Brunn; Jarrod E Jones; James N Wygant; Bradley W McIntyre; William T Butler |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: European journal of oral sciences Volume: 112 ISSN: 0909-8836 ISO Abbreviation: Eur. J. Oral Sci. Publication Date: 2004 Apr |
Date Detail:
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Created Date: 2004-04-01 Completed Date: 2004-05-19 Revised Date: 2010-02-05 |
Medline Journal Info:
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Nlm Unique ID: 9504563 Medline TA: Eur J Oral Sci Country: Denmark |
Other Details:
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Languages: eng Pagination: 163-70 Citation Subset: D; IM |
Affiliation:
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Department of Endodontics and Periodontics, The University of Texas-Houston Health Science Center Dental Branch, Houston, Texas, USA. Oto.Baba@uth.tmc.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alveolar Process
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cytology Ameloblasts / cytology Animals Dental Cementum / cytology Extracellular Matrix / chemistry Fibroblasts / cytology Odontoblasts / cytology Odontogenesis / physiology Osteoblasts / cytology Osteocytes / cytology Periodontal Ligament / cytology Periodontium / cytology* Phosphoproteins / analysis Protein Precursors / analysis Rats Rats, Sprague-Dawley Sialoglycoproteins / analysis* Tooth Root / cytology Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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DE05092/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phosphoproteins; 0/Protein Precursors; 0/Sialoglycoproteins; 0/dentin sialophosphoprotein; 0/phosphophoryn |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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