| Detection of cytotoxic anti-LEDGF autoantibodies in atopic dermatitis. | |
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MedLine Citation:
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PMID: 12515286 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the last two decades, atopic dermatitis (AD) has been of increasing clinical significance in Japan. Eight-20% of patients with AD developed progressive cataracts (cataract-AD) and lens epithelial cells (LECs) were severely damaged. Lens epithelium-derived growth factor (LEDGF) is a newly isolated survival factor. In the presence of LEDGF, LECs survive well and in the absence of LEDGF, they become highly susceptible to stress. We investigated (1) whether auto-antibody (auto-Ab) to LEDGF is present in sera of AD patients and (2) whether depletion of LEDGF by the auto-Ab kills LECs. In sera from 26 patients with AD using ELISA, we found significantly higher levels of auto-Ab to LEDGF than that in a normal control group. Affinity purified auto-Ab to LEDGF from these sera killed LECs without complement activation. Levels of histamine in the AD group were significantly higher and levels of prostaglandin E2 were significantly lower than in the normal group. However, statistically there are no differences between sera from AD and cataract-AD in levels of Ab to LEDGF, histamine, prostaglandin E2 (PGE2), immunoglobulin E (IgE) and eosinophiles. We speculate that cataract-AD may be induced, in part, by a combination of high levels of serum histamine and eye rubbing which could break the blood-aqueous barrier to allow the entry of Ab to LEDGF into the privileged compartment, thus, reducing LEDGF levels, resulting in damage to LECs, and cataract formation. |
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Authors:
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Masahiko Ayaki; Nobuyuki Ohoguro; Noriyuki Azuma; Yoshinao Majima; Kiyomi Yata; Nobuhiro Ibaraki; Dhirendra P Singh; Vincent Ko; Toshimichi Shinohara |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Autoimmunity Volume: 35 ISSN: 0891-6934 ISO Abbreviation: Autoimmunity Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2003-01-07 Completed Date: 2003-05-02 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8900070 Medline TA: Autoimmunity Country: England |
Other Details:
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Languages: eng Pagination: 319-27 Citation Subset: IM |
Affiliation:
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Center for Ophthalmic Research, Brigham and Women's Hospital, Department of Ophthalmology, Harvard Medical School, 221 Longwood Avenue, Boston, MA 02115, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoantibodies / blood* Case-Control Studies Cataract / blood, complications, immunology Cytotoxicity, Immunologic Dermatitis, Atopic / blood, complications, immunology* Dinoprostone / blood Eosinophils Epithelial Cells / immunology Histamine / blood Humans Immunoglobulins / blood Intercellular Signaling Peptides and Proteins / immunology* Lens, Crystalline / immunology Mice |
| Grant Support | |
ID/Acronym/Agency:
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EY10958/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Autoantibodies; 0/Immunoglobulins; 0/Intercellular Signaling Peptides and Proteins; 0/lens epithelium-derived growth factor; 363-24-6/Dinoprostone; 51-45-6/Histamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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