| Detection of cytochrome P450 gene expression in human placenta in first trimester of pregnancy. | |
| | |
MedLine Citation:
|
PMID: 8694864 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Human first-trimester placentas were screened for the expression of xenobiotic-metabolizing cytochrome P450 (CYP) genes. mRNAs of CYP1A1, CYP1A2, CYP2C, CYP2D6, CYP2E1, CYP2F1, CYP3A4, CYP3A5, CYP3A7, and CYP4B1 were identified by reverse transcriptase-polymearse chain reaction (RT-PCR) in at least some of the six placental samples studied. CYP2A and CYP2B message were absent in all samples. The level of all of these CYP mRNAs was lower compared to the corresponding levels in liver or lung. the catalytic activity marker (7-ethoxyresorufin O-deethylase) was inducible in the placentas by maternal cigarette smoking. Thus, the regulatory system of placental CYP1A1, mediated by the Ah-receptor, appears to be developed as early as the first trimester of pregnancy. Three immunoreactive bands from placental microsomes were detected by an antihuman CYP3A4 antibody, but no functional activity of CYP3A enzymes could be detected. These results show that placental tissue during the first trimester of pregnancy has the potential of expressing several CYP genes, and forms a basis for subsequent analysis of these forms at the protein and functional level. |
| | |
Authors:
|
J Hakkola; H Raunio; R Purkunen; O Pelkonen; S Saarikoski; T Cresteil; M Pasanen |
Related Documents
:
|
7503724 - Constitutive expression of the thrombopoietin gene in a human hepatoma cell line. 14770174 - Car/pxr provide directives for cyp3a41 gene regulation differently from cyp3a11. 20966044 - Cigarette smoke extract induces cyp2b6 through constitutive androstane receptor in hepa... 14560034 - Agonist and chemopreventative ligands induce differential transcriptional cofactor recr... 21464834 - α(1)-fetoprotein transcription factor (ftf)/liver receptor homolog-1 (lrh-1) is an ess... 17356014 - Extended polyglutamine repeats trigger a feedback loop involving the mitochondrial comp... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochemical pharmacology Volume: 52 ISSN: 0006-2952 ISO Abbreviation: Biochem. Pharmacol. Publication Date: 1996 Jul |
Date Detail:
|
Created Date: 1996-08-26 Completed Date: 1996-08-26 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0101032 Medline TA: Biochem Pharmacol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 379-83 Citation Subset: IM |
Affiliation:
|
Department of Pharmacology and Toxicology, University of Oulu, Finland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Base Sequence Cytochrome P-450 Enzyme System / biosynthesis*, genetics Female Gene Expression Humans Isoenzymes / biosynthesis*, genetics Microsomes / enzymology Molecular Sequence Data Placenta / enzymology* Polymerase Chain Reaction Pregnancy Pregnancy Trimester, First RNA, Messenger / analysis |
| Chemical | |
Reg. No./Substance:
|
0/Isoenzymes; 0/RNA, Messenger; 9035-51-2/Cytochrome P-450 Enzyme System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Use of inhibitors of ion transport to differentiate iron transporters in erythroid cells.
Next Document: Characterization and identification of a human dentin phosphophoryn.