| Detection and correction of a migration anomaly on a 310 genetic analyzer. | |
| | |
MedLine Citation:
|
PMID: 11110196 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
During STR analysis on the 310 Genetic Analyzer, retarded migration of GS500ROX size standards and alleles in some samples was observed. The contribution of reagents, capillary and performance optimized polymer POP 4 to the observed anomaly was experimentally eliminated. Variation in electrophoresis temperature between 55 degrees C and 65 degrees C did not alter the rate of migration of GX500ROX size standard and sample alleles. An eroded connector for the cathode mounted on the heat plate assembly caused the abnormal migration. Hence, it is important to verify the mobility of all fragments in the size standard for each sample to avoid any erroneous allele calls by the automated data analysis software. |
| | |
Authors:
|
J G Shewale; S L Richey; S K Sinha |
Related Documents
:
|
16382826 - Informativeness of the codis str loci for admixture analysis. 22199116 - Variants in the 3' untranslated region of the kcnq1-encoded kv7.1 potassium channel mod... 1720926 - A cluster of highly polymorphic dinucleotide repeats in intron 17b of the cystic fibros... 7918076 - Haemophilia a diagnosis by simultaneous analysis of two variable dinucleotide tandem re... 9032656 - Gaucher disease: molecular screening of the glucocerebrosidase 1601g and 1601a alleles ... 3280976 - Dna sequence analysis of spontaneous mutations in the sup4-o gene of saccharomyces cere... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of forensic sciences Volume: 45 ISSN: 0022-1198 ISO Abbreviation: J. Forensic Sci. Publication Date: 2000 Nov |
Date Detail:
|
Created Date: 2000-12-11 Completed Date: 2001-01-04 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0375370 Medline TA: J Forensic Sci Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1339-42 Citation Subset: IM |
Affiliation:
|
ReliaGene Technologies, Inc., New Orleans, LA 70123, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
DNA Fingerprinting* Electrophoresis, Capillary Forensic Medicine / methods Humans Polymerase Chain Reaction Reference Standards Reproducibility of Results Tandem Repeat Sequences / genetics* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Metastatic calcification of the cardiac conduction system with heart block: an under-reported entity...
Next Document: Ecological risks of diazinon from agricultural use in the Sacramento-San Joaquin River Basins, Calif...