Document Detail


Detection and confirmation of Clostridium botulinum in water used for cooling at a plant producing low-acid canned foods.
MedLine Citation:
PMID:  20889791     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Our laboratory tested water samples used for cooling low-acid canned foods at a canning facility under investigation by the U.S. Food and Drug Administration. We used an enzyme-linked immunosorbent assay with digoxigenin-labeled antibodies (DIG-ELISA) and real-time PCR as screening methods and confirmed the presence of neurotoxin-producing Clostridium botulinum in the samples by mouse bioassay.
Authors:
Amita Sachdeva; Stephanie L H Defibaugh-Chávez; James B Day; Donald Zink; Shashi K Sharma
Publication Detail:
Type:  Journal Article     Date:  2010-10-01
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  76     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-08     Completed Date:  2011-02-22     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7653-7     Citation Subset:  IM    
Affiliation:
FDA, Center for Food Safety and Applied Nutrition, College Park, MD 20740, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteriological Techniques / methods
Botulinum Toxins / biosynthesis
Botulism / diagnosis,  pathology
Clostridium botulinum / isolation & purification*
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay / methods
Food Industry / methods*
Food, Preserved*
Mass Screening / methods
Mice
Polymerase Chain Reaction / methods
United States
Water Microbiology*
Chemical
Reg. No./Substance:
EC 3.4.24.69/Botulinum Toxins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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