| Detection of clinically relevant levels of protein analyte under physiologic buffer using planar field effect transistors. | |
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MedLine Citation:
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PMID: 18632260 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Electrochemical detection of protein binding at physiological salt concentration by planar field effect transistor platforms has yet to be documented convincingly. Here we report detection of streptavidin and clinically relevant levels of biotinylated monokine induced by interferon gamma (MIG) at physiological salt concentrations with AlGaN heterojunction field effect transistors (HFETs). The AlGaN HFETs are functionalized with a silane linker and analyte-specific affinity elements. Polarity of sensor responses is as expected from n-type HFETs to negatively and positively charged analytes. Sensitivity of the HFET sensors increases when salt concentration decreases, and the devices also exhibit dose-dependent responses to analyte. Detection of clinically relevant MIG concentrations at physiological salt levels demonstrates the potential for AlGaN devices to be used in development of in vivo biosensors. |
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Authors:
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Samit Gupta; Mark Elias; Xuejin Wen; John Shapiro; Leonard Brillson; Wu Lu; Stephen Craig Lee |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-06-08 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 24 ISSN: 1873-4235 ISO Abbreviation: Biosens Bioelectron Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-10-20 Completed Date: 2009-02-12 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
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Languages: eng Pagination: 505-11 Citation Subset: IM |
Affiliation:
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The Department of Biomedical Engineering, The Ohio State University, Columbus, OH, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biosensing Techniques
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instrumentation*,
methods Chemokine CXCL9 / analysis*, chemistry Electrochemistry / instrumentation*, methods Equipment Design Equipment Failure Analysis Hydrogen-Ion Concentration Immunoassay / instrumentation*, methods Microelectrodes Protein Interaction Mapping / instrumentation*, methods Reproducibility of Results Sensitivity and Specificity Streptavidin / analysis*, chemistry Transistors, Electronic* |
| Grant Support | |
ID/Acronym/Agency:
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EB004960/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemokine CXCL9; 9013-20-1/Streptavidin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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