Document Detail


Detection of clinically relevant levels of protein analyte under physiologic buffer using planar field effect transistors.
MedLine Citation:
PMID:  18632260     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Electrochemical detection of protein binding at physiological salt concentration by planar field effect transistor platforms has yet to be documented convincingly. Here we report detection of streptavidin and clinically relevant levels of biotinylated monokine induced by interferon gamma (MIG) at physiological salt concentrations with AlGaN heterojunction field effect transistors (HFETs). The AlGaN HFETs are functionalized with a silane linker and analyte-specific affinity elements. Polarity of sensor responses is as expected from n-type HFETs to negatively and positively charged analytes. Sensitivity of the HFET sensors increases when salt concentration decreases, and the devices also exhibit dose-dependent responses to analyte. Detection of clinically relevant MIG concentrations at physiological salt levels demonstrates the potential for AlGaN devices to be used in development of in vivo biosensors.
Authors:
Samit Gupta; Mark Elias; Xuejin Wen; John Shapiro; Leonard Brillson; Wu Lu; Stephen Craig Lee
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-06-08
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  24     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-10-20     Completed Date:  2009-02-12     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  505-11     Citation Subset:  IM    
Affiliation:
The Department of Biomedical Engineering, The Ohio State University, Columbus, OH, USA.
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MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques / instrumentation*,  methods
Chemokine CXCL9 / analysis*,  chemistry
Electrochemistry / instrumentation*,  methods
Equipment Design
Equipment Failure Analysis
Hydrogen-Ion Concentration
Immunoassay / instrumentation*,  methods
Microelectrodes
Protein Interaction Mapping / instrumentation*,  methods
Reproducibility of Results
Sensitivity and Specificity
Streptavidin / analysis*,  chemistry
Transistors, Electronic*
Grant Support
ID/Acronym/Agency:
EB004960/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Chemokine CXCL9; 9013-20-1/Streptavidin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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