Document Detail

Detection and clinical significance of gene rearrangements in Chinese adult acute lymphoblastic leukemia patients.
MedLine Citation:
PMID:  23210573     Owner:  NLM     Status:  Publisher    
ABSTRACT This study aimed to develop a novel multiplex reverse transcription-nested polymerase chain reaction (RT-nPCR) assay to accurately and effectively detect 10 common gene rearrangements in adult acute lymphoblastic leukemia (ALL) and to examine the clinicopathologic characteristics and other genetic aberrations of ALL patients expressing different fusion genes. Our RT-nPCR assay had a positive detection rate of 35.15% (90/256) for the 10 fusion genes. BCR-ABL1, FUS-ERG, MLL-AF4, ETV6-RUNX1, E2A-PBX1, dupMLL, MLL-AF10, MLL-ENL, SET-NUP214, and SIL-TAL1 were detected in 36 (14.06%), 14 (5.47%), 14 (5.47%), 4 (1.56%), 4 (1.56%), 5 (1.95%), 4 (1.56%), 2 (0.78%), 2 (0.78%), and 5 patients (1.95%) respectively. The RT-nPCR results were further confirmed by split-out PCR, and cytogenetic and fluorescent in situ hybridization (FISH) analysis revealed corresponding translocations and fusions in 63 and 74 cases, respectively. JAK2 and IKZF1 mutations were commonly detected in BCR-ABL1 ALL patients, and HOX overexpression was highly correlated with MLL fusions and SET-NUP214. This study demonstrates that RT-nPCR is an effective method for identifying 10 gene rearrangements in adult ALL, and it could potentially be developed for diagnostic use and prognostic studies of ALL.
Fang Liu; Li Gao; Yu Jing; Yuan-Yuan Xu; Yi Ding; Min-Hang Zhou; Chao Ma; Mian-Yang Li; Jun-Zhong Sun; Li-Li Wang; Li Yu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-5
Journal Detail:
Title:  Leukemia & lymphoma     Volume:  -     ISSN:  1029-2403     ISO Abbreviation:  Leuk. Lymphoma     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9007422     Medline TA:  Leuk Lymphoma     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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