Document Detail

Detection of chromosomal abnormalities of chromosome 12 in uterine leiomyoma using fluorescence in situ hybridization.
MedLine Citation:
PMID:  8914635     Owner:  NLM     Status:  MEDLINE    
Fifty uterine leiomyomas were examined using conventional cytogenetic method and fluorescence in situ hybridization (FISH) for detection of chromosomal abnormalities of chromosome 12. Of the 50 tumors, nine were examined using FISH on the non-cultured samples. Two (4.0%) of 50 tumor samples examined showed chromosomal abnormalities of chromosome 12 by the conventional cytogenetic analysis. For FISH, the whole-chromosome painting probe and D12Z3 probe specific for the centromeric region were used. Of the 50 cultured samples, 10 showed structural aberrations and four showed numerical aberrations of chromosome 12 by FISH analysis. Of the nine non-cultured samples, four showed structural abnormalities of chromosome 12, all of which also showed structural abnormalities of chromosome 12 on the cultured samples. These results indicate that chromosomal abnormalities of chromosome 12 are important in the biology of at least some types of uterine leiomyoma, and that FISH is a useful complement to the conventional cytogenetic analysis in the study of solid tumors.
S Hayashi; N Miharu; E Okamoto; O Samura; T Hara; K Ohama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Japanese journal of human genetics     Volume:  41     ISSN:  0916-8478     ISO Abbreviation:  Jpn. J. Hum. Genet.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-12-17     Completed Date:  1996-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9213239     Medline TA:  Jpn J Hum Genet     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  193-202     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Hiroshima University School of Medicine, Japan.
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MeSH Terms
Chromosome Aberrations*
Chromosome Disorders*
Chromosome Mapping
Chromosomes, Human, Pair 12*
Genetic Markers
In Situ Hybridization, Fluorescence
Leiomyoma / genetics*,  pathology
Uterine Neoplasms / genetics*,  pathology
Reg. No./Substance:
0/Genetic Markers

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