Document Detail


Detection and characterization of ubiquitylated H2B in mammalian cells.
MedLine Citation:
PMID:  21439383     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Histone H2B ubiquitylation was shown to be associated with actively transcribed genes in mammalian cells and has been suggested to be involved in transcriptional regulation. Despite the limited applicability of genetic tools to analyze H2B ubiquitylation in mammals, several biochemical and immunological approaches have been successfully implemented to study this modification. Here we describe several techniques to detect ubiquitylated H2B in mammalian cells and to dissect its genomic localization.
Authors:
Efrat Shema; Moshe Oren; Neri Minsky
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Publication Detail:
Type:  Journal Article     Date:  2011-03-23
Journal Detail:
Title:  Methods (San Diego, Calif.)     Volume:  54     ISSN:  1095-9130     ISO Abbreviation:  Methods     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-20     Completed Date:  2011-09-30     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  9426302     Medline TA:  Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  326-30     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel. efrat.shema@weizmann.ac.il
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MeSH Terms
Descriptor/Qualifier:
Analytic Sample Preparation Methods
Animals
Antibodies, Monoclonal / biosynthesis,  chemistry
Blotting, Western
Cell Extracts / chemistry
Cell Fusion
Cell Nucleus / chemistry
Cells, Cultured
Chromatin / chemistry,  metabolism
Chromatin Immunoprecipitation
Female
Histones / chemistry,  isolation & purification*,  metabolism
Hybridomas
Mice
Mice, Inbred BALB C
Peptide Fragments / chemistry
Recombinant Fusion Proteins / chemistry,  isolation & purification*,  metabolism
Ubiquitinated Proteins / chemistry,  isolation & purification*,  metabolism
Grant Support
ID/Acronym/Agency:
R37 CA040099-27/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Cell Extracts; 0/Chromatin; 0/Histones; 0/Peptide Fragments; 0/Recombinant Fusion Proteins; 0/Ubiquitinated Proteins
Comments/Corrections

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