| Detection and characterization of ubiquitylated H2B in mammalian cells. | |
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MedLine Citation:
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PMID: 21439383 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Histone H2B ubiquitylation was shown to be associated with actively transcribed genes in mammalian cells and has been suggested to be involved in transcriptional regulation. Despite the limited applicability of genetic tools to analyze H2B ubiquitylation in mammals, several biochemical and immunological approaches have been successfully implemented to study this modification. Here we describe several techniques to detect ubiquitylated H2B in mammalian cells and to dissect its genomic localization. |
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Authors:
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Efrat Shema; Moshe Oren; Neri Minsky |
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Publication Detail:
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Type: Journal Article Date: 2011-03-23 |
Journal Detail:
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Title: Methods (San Diego, Calif.) Volume: 54 ISSN: 1095-9130 ISO Abbreviation: Methods Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-06-20 Completed Date: 2011-09-30 Revised Date: 2012-09-18 |
Medline Journal Info:
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Nlm Unique ID: 9426302 Medline TA: Methods Country: United States |
Other Details:
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Languages: eng Pagination: 326-30 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel. efrat.shema@weizmann.ac.il |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Analytic Sample Preparation Methods Animals Antibodies, Monoclonal / biosynthesis, chemistry Blotting, Western Cell Extracts / chemistry Cell Fusion Cell Nucleus / chemistry Cells, Cultured Chromatin / chemistry, metabolism Chromatin Immunoprecipitation Female Histones / chemistry, isolation & purification*, metabolism Hybridomas Mice Mice, Inbred BALB C Peptide Fragments / chemistry Recombinant Fusion Proteins / chemistry, isolation & purification*, metabolism Ubiquitinated Proteins / chemistry, isolation & purification*, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R37 CA040099-27/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Cell Extracts; 0/Chromatin; 0/Histones; 0/Peptide Fragments; 0/Recombinant Fusion Proteins; 0/Ubiquitinated Proteins |
| Comments/Corrections | |
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