Document Detail


Detection and characterization of two cytotoxins produced by Campylobacter jejuni strains.
MedLine Citation:
PMID:  9728413     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Campylobacter jejuni strains are able to produce at least two different cytotoxins called "cytolethal distending toxin" (CLDT) and "cytolethal rounding toxin" (CLRT). In this study, we investigated the corresponding changes in CHO-K1 cells using the cell counter and analyzer system CASY 1. Determination of the cell volume after toxin treatment of the cells is a useful criterion for differentiation between the cytotoxic activities produced by Campylobacter strains. Incubation of the cells with crude CLDT resulted in a decrease in the cell count combined with a dramatic increase of the mean cell volume in comparison to the control culture. A decrease in the cell count was also seen as a response to CLRT preparations, while this toxin had no effect on the mean cell volume determined. It was shown that only CLDT caused histone-associated DNA fragments in the cytoplasm of CHO-K1 cells indicating an apoptotic pathway of cell death. In addition, the polymerase chain reaction (PCR) was employed to screen Campylobacter strains for the presence of the cdtB gene sequence, which was detectable in all strains investigated.
Authors:
I Hänel; F Schulze; H Hotzel; E Schubert
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Zentralblatt für Bakteriologie : international journal of medical microbiology     Volume:  288     ISSN:  0934-8840     ISO Abbreviation:  Zentralbl. Bakteriol.     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-11-05     Completed Date:  1998-11-05     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9203851     Medline TA:  Zentralbl Bakteriol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  131-43     Citation Subset:  IM    
Affiliation:
Federal Institute for Health Protection of Consumers and Veterinary Medicine, Division 4: Bacterial Animal Diseases and Zoonoses Control, Jena, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Toxins / analysis,  genetics,  isolation & purification,  toxicity*
CHO Cells
Campylobacter fetus / genetics,  growth & development,  metabolism,  pathogenicity*
Campylobacter jejuni / genetics,  growth & development,  metabolism,  pathogenicity*
Cricetinae
Cytotoxins / analysis,  genetics,  isolation & purification,  toxicity*
DNA, Bacterial / analysis,  isolation & purification
Genes, Bacterial
Humans
Polymerase Chain Reaction / methods
Virulence
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/Cytotoxins; 0/DNA, Bacterial; 0/cytolethal distending toxin

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