Document Detail


Detection and characterization of plasmids in Pseudomonas aeruginosa strain PAO.
MedLine Citation:
PMID:  4633347     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using a variety of techniques, it has been established that the sex factor FP2 has a density of 1.717 g/cm(3) (corresponding to a guanine plus cytosine [G + C] content of 58%) and a mean circular contour length of 28.5 +/- 0.6 mum (corresponding to a molecular weight of 59 x 10(6)). Another sex factor FP39 has a density of 1.719 g/cm(3) (corresponding to a G + C content of 60%) and a mean circular contour length of 26.5 +/- 0.5 mum (corresponding to a molecular weight of 55 x 10(6)). It appears that all, or nearly all, of the FP sex factor deoxyribonucleic acid occurs as covalent circular molecules under the conditions employed. In addition, these procedures have been used to demonstrate that strain PAO, a naturally occurring female (i.e., FP(-)) strain of Pseudomonas aeruginosa, harbors a number of cryptic plasmids having similar densities to the bulk of the cell deoxyribonucleic acid (1.726 g/cm(3)) and occurring as covalent circular molecules.
Authors:
J M Pemberton; A J Clark
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  114     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1973 Apr 
Date Detail:
Created Date:  1973-06-19     Completed Date:  1973-06-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  424-33     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenine / metabolism
Bromides
Centrifugation, Density Gradient
Cesium
Chlorides
DNA, Bacterial / isolation & purification
DNA, Circular / isolation & purification
Ethidium
Extrachromosomal Inheritance*
Microscopy, Electron
Mutation
Pseudomonas aeruginosa* / analysis*
Tritium
Chemical
Reg. No./Substance:
0/Bromides; 0/Chlorides; 0/DNA, Bacterial; 0/DNA, Circular; 10028-17-8/Tritium; 3546-21-2/Ethidium; 73-24-5/Adenine; 7440-46-2/Cesium
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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