| Detection and characterization of four novel genotypes of Ehrlichia canis from dogs. | |
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MedLine Citation:
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PMID: 20451333 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The genetic diversity of Ehrlichia canis strains worldwide is currently poorly defined. The present study aimed to characterize E. canis strains in naturally infected dogs in Taiwan, using a combination of PCR and sequence analysis of the 16S rDNA and two antigen-encoding genes, gp19 and gp36. Genomic DNA was extracted from 34 parasitemic dogs and the genes of the pathogen were separately amplified, sequenced, and aligned with corresponding sequences available in GenBank. All 16S rDNA sequences (1623 bp) amplified from the Taiwanese isolates were identical and had very high similarity (99.4-100%) with previously reported E. canis sequences. Nevertheless, most of the gp19 gene sequences (414 bp) from the Taiwanese isolates had three specific nucleotide substitutions at positions 9, 323 and 371 that resulted in three amino acid changes. The gp36 gene of the Taiwanese isolates consists of three regions: a 5' end pre-repeat region (426 bp), a tandem repeat region with variable numbers of the 27-bp repeat unit depending on the isolate, and a 3' end region (87 bp). The nucleotide sequences of the 5' end region of gp36 from Taiwanese isolates were identical to each other, but unexpectedly, quite distinct from the sequences of eleven other E. canis strains previously published, with 86.7-87.2% identities only. A phylogenetic tree of E. canis strains based on the gp36 amino acid sequences showed that the Taiwanese isolates fell into a separate clade, indicating the presence of a novel strain that had not yet been characterized. |
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Authors:
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Yu-Chen Hsieh; Chung-Chan Lee; Chau-Loong Tsang; Yang-Tsung Chung |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-21 |
Journal Detail:
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Title: Veterinary microbiology Volume: 146 ISSN: 1873-2542 ISO Abbreviation: Vet. Microbiol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7705469 Medline TA: Vet Microbiol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 70-5 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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