Document Detail

Detection of cerebral spinal fluid-associated chemokines in birth traumatized premature babies by chip-based immunoaffinity CE.
MedLine Citation:
PMID:  23512340     Owner:  NLM     Status:  MEDLINE    
A major concern in treating premature infants with birth-associated head trauma is the rapid determination of reliable biomarkers of neuroinflammation. To this end a chip-based immunoaffinity CE device has been applied to determine the concentrations of inflammation-associated chemokines in samples of cerebral spinal fluid collected from such subjects. The chip utilizes replaceable immunoaffinity disks, to which reactive antibody fragments (FAb) of six antichemokine-specific antibodies were immobilized. Following injection of a sample into the device, the analytes were captured by the immobilized FAbs, labeled in situ with a red laser dye, chemically released and separated by CE. Each resolved peak was measured on-line by LIF detection and the results compared to standard curves produced by running known chemokine standards through the immunoaffinity system. The complete processing of a sample took 10 min with separation of all six analytes being achieved in less than 2 min. The system compared well to commercial ELISA, analysis of the results by linear regression demonstrating r(2) values in the range of 0.903-0.978, and intra and interassay CV of the migration times and the measured peak areas being less than 2.3 and 5%, respectively. Application of the system to analysis of cerebrospinal fluid from head traumatized babies clearly indicated the group with mild trauma versus those with severe injury. Additionally, CE analysis demonstrated that the severe trauma group could be divided into individuals with good and poor prognosis, which correlated with the clinical finding for each patient.
Terry M Phillips; Edward Wellner
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2013-05-10
Journal Detail:
Title:  Electrophoresis     Volume:  34     ISSN:  1522-2683     ISO Abbreviation:  Electrophoresis     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-06-05     Completed Date:  2013-10-22     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1530-8     Citation Subset:  IM    
Copyright Information:
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Chemokines / cerebrospinal fluid*
Craniocerebral Trauma / cerebrospinal fluid*
Electrophoresis, Microchip / instrumentation*
Enzyme-Linked Immunosorbent Assay
Equipment Design
Infant, Newborn
Infant, Newborn, Diseases / cerebrospinal fluid*
Infant, Premature
Inflammation / cerebrospinal fluid
Grant Support
Reg. No./Substance:

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