Document Detail

Detection of a cephalosporin C acetyl esterase in the carbamate cephalosporin antibiotic-producing culture, Streptomyces clavuligerus.
MedLine Citation:
PMID:  5045469     Owner:  NLM     Status:  MEDLINE    
The possible role of a cephalosporin C acetyl esterase in the formation of the beta-lactam antibiotic A16886A, 7-(5-amino-5-carboxyvaleramido)-3-carbamoyloxy-methyl-3-cephem-4-carboxylic acid, by Streptomyces clavuligerus was studied. Addition of dl-valine-(14)C-1 to a fermentation of Cephalosporium acremonium gave cephalosporin C-(14)C-9 (I). The formation of deacetylcephalosporin C-(14)C-9 from I occurred at a greater rate in broths of S. clavuligerus than in the broths of C. acremonium, or in the autoclaved broths of S. clavuligerus. Diisopropylfluorophosphate partially inhibited the deacetylation of I in S. clavuligerus broth. An intracellular cephalosporin C esterase in S. clavuligerus could not be demonstrated. Although A16886A has been chemically synthesized from deacetylcephalosporin C, this reaction could not be demonstrated with S. clavuligerus.
D R Brannon; D S Fukuda; J A Mabe; F M Huber; J G Whitney
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  1     ISSN:  0066-4804     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  1972 Mar 
Date Detail:
Created Date:  1972-09-19     Completed Date:  1972-09-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  237-41     Citation Subset:  IM    
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MeSH Terms
Acetylesterase / metabolism*
Carbamates / biosynthesis
Carbon Isotopes
Cephalosporins / biosynthesis*
Culture Media
Depression, Chemical
Isoflurophate / pharmacology
Methyl Ethers / biosynthesis
Osmolar Concentration
Streptomyces / drug effects,  enzymology*,  metabolism
Reg. No./Substance:
0/Carbamates; 0/Carbon Isotopes; 0/Cephalosporins; 0/Culture Media; 0/Methyl Ethers; 55-91-4/Isoflurophate; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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