Document Detail

Detection of cell-cycle regulators in failed arteriovenous fistulas for haemodialysis.
MedLine Citation:
PMID:  12637654     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Chronic renal failure (CRF) patients rely on arteriovenous fistulas (AVFs) for haemodialysis vascular access. Intimal hyperplastic stenoses result in failure of AVFs and frequent intervention is required to maintain vascular access. The extent of intimal hyperplasia depends on the interplay between cyclins and cyclin-dependent kinases (e.g. cdk2), positively regulating cell-cycle progression. cdk activity is negatively modulated by the interaction with cdk inhibitory proteins, such as p21(Waf1) and p27(Kip1). Little is known about the expression of these proteins in the development of intimal hyperplasia in AVFs. METHODS: p21(Waf1), p27(Kip1), cdk2 and Proliferating Cell Nuclear Antigen immunoreactivity was determined in 18 failed AVFs from 16 CRF patients and 10 non-diseased vessels (five arteries and five veins). RESULTS: The percentage of p21(Waf1)-positive cells was significantly lower in AVFs (3+/-1%), compared with normal veins and arteries (62+/-4 and 63+/-4%, respectively; P<0.001). cdk2-positive cells were significantly higher in AVFs (40.7+/-3.7%) than in normal veins and arteries (2+/-1 and 0+/-0%, respectively; P<0.001). Although no difference in p27(Kip1) immunoreactivity was found between AVFs (37+/-17%) and veins (23+/-8%; P=0.208), it was lower in healthy arteries (17+/-11%; P=0.037). CONCLUSIONS: The data suggest that in failed AVFs, p21(Waf1), but not p27(Kip1), is related to intimal hyperplasia. This is the first report to show involvement of cell-cycle regulators in AVF-related human intimal hyperplasia.
Rick de Graaf; Ruben Dammers; Tryfon Vainas; Arnold P G Hoeks; Jan H M Tordoir
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association     Volume:  18     ISSN:  0931-0509     ISO Abbreviation:  Nephrol. Dial. Transplant.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-03-14     Completed Date:  2003-11-25     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8706402     Medline TA:  Nephrol Dial Transplant     Country:  England    
Other Details:
Languages:  eng     Pagination:  814-8     Citation Subset:  IM    
Department of Surgery, University Hospital Maastricht, Maastricht, The Netherlands.
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MeSH Terms
Analysis of Variance
Arteriovenous Shunt, Surgical / adverse effects*,  methods
Biological Markers / analysis
Biopsy, Needle
Cell Cycle Proteins / analysis*,  metabolism
Constriction, Pathologic
Cyclin-Dependent Kinases / analysis,  metabolism
Hyperplasia / pathology
Kidney Failure, Chronic / therapy
Middle Aged
Renal Dialysis / adverse effects,  methods*
Sensitivity and Specificity
Tunica Intima / pathology*
Reg. No./Substance:
0/Biological Markers; 0/Cell Cycle Proteins; EC Kinases

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