| Detection of caspase-3 activation in single cells by fluorescence resonance energy transfer during photodynamic therapy induced apoptosis. | |
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MedLine Citation:
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PMID: 15958279 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In many apoptosis pathways, activation of caspase-3 is considered the final stage. In this study, we studied PDT induced caspase-3 activation with fluorescence resonance energy transfer (FRET) technique. A recombinant caspase-3 substrate, SCAT3, was used as the FRET probe. FRET fluorescence images were collected after PDT or TNF-alpha induced apoptosis. By analyzing the dynamic changes of FRET fluorescence, the results indicate that the caspase-3 activation started immediately after the PDT treatment. In contrast, FRET disruption caused by caspase-3 activation started at 3h after TNF-alpha treatment, due to different signaling pathway. The results have proofed, for the first time, that FRET is a sensitive technique that can be used to investigate PDT-induced activation of caspase-3 in real-time and in single cells. By choosing appropriate recombinant substrates as FRET probes, it is likely that FRET technique will provide a new real-time means to study the mechanism of PDT at single cell level. |
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Authors:
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Yunxia Wu; Da Xing; Shiming Luo; Yonghong Tang; Qun Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-06-14 |
Journal Detail:
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Title: Cancer letters Volume: 235 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-04-24 Completed Date: 2006-06-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 239-47 Citation Subset: IM |
Affiliation:
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Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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drug therapy,
enzymology,
pathology Apoptosis / drug effects* Caspase 3 Caspases / metabolism* Cell Line, Tumor Enzyme Activation Fluorescence Resonance Energy Transfer Humans Lung Neoplasms / drug therapy, enzymology, pathology Photochemotherapy* Tumor Necrosis Factor-alpha / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Tumor Necrosis Factor-alpha; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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