Document Detail


Detection of cancer stem cells from the C6 glioma cell line.
MedLine Citation:
PMID:  19383245     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Various malignant cancers have been found to contain a sub-population of stem cell-like tumour cells, or cancer stem cells (CSCs), however, culture methods for CSCs and the size of the fraction of CSCs in C6, which is a commonly used glioma cell line, remain controversial. In this study, we demonstrated that the C6 cell line contains a fraction of tumour cells that can form tumour spheres in a simplified serum-free neural stem cell medium and express CD133 and nestin, which are widely-used markers for brain CSCs. Immunohistochemistry and immunofluorescence confirmed the existence of CSCs both in the C6 cell line and C6 xenografts. Flow cytometry demonstrated that 4.02% of cells in the C6 cell line and 4.21% in the C6 xenografts presented as CSCs. These results confirm the fraction of CSCs in the C6 cell line and provide a simple and effective method for isolation of CSCs to study the initiation and progression of human glioma and, possibly, other malignant tumours.
Authors:
X-D Zhou; X-Y Wang; F-J Qu; Y-H Zhong; X-D Lu; P Zhao; D-H Wang; Q-B Huang; L Zhang; X-G Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of international medical research     Volume:  37     ISSN:  0300-0605     ISO Abbreviation:  J. Int. Med. Res.     Publication Date:    2009 Mar-Apr
Date Detail:
Created Date:  2009-04-22     Completed Date:  2009-06-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0346411     Medline TA:  J Int Med Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  503-10     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Shandong University, Qilu Hospital, Jinan, Shandong, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD / metabolism
Cell Proliferation
Flow Cytometry
Fluorescent Antibody Technique
Glioma / pathology*
Glycoproteins / metabolism
Intermediate Filament Proteins / metabolism
Mice
Mice, Nude
Neoplastic Stem Cells / pathology*
Nerve Tissue Proteins / metabolism
Peptides / metabolism
Rats
Spheroids, Cellular / pathology
Tumor Cells, Cultured
Tumor Markers, Biological / metabolism
Xenograft Model Antitumor Assays
Chemical
Reg. No./Substance:
0/AC133 antigen; 0/Antigens, CD; 0/Glycoproteins; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/Peptides; 0/Tumor Markers, Biological; 0/nestin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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