Document Detail


Detection of cancer cells using a peptide nanotube-folic acid modified graphene electrode.
MedLine Citation:
PMID:  23150875     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This article describes the preparation of a graphene electrode modified with a new conjugate of peptide nanotubes and folic acid for the selective detection of human cervical cancer cells over-expressing folate receptors. The functionalization of peptide nanotubes with folic acid was confirmed by fluorescence microscopy and atomic force microscopy. The peptide nanotube-folic acid modified graphene electrode was characterized by scanning electron microscopy and cyclic voltammetry. The modification of the graphene electrode with peptide nanotube-folic acid led to an increase in the current signal. The human cervical cancer cells were bound to the modified electrode through the folic acid-folate receptor interaction. Cyclic voltammograms in the presence of [Fe(CN)(6)](3-/4-) as a redox species demonstrated that the binding of the folate receptor from human cervical cancer cells to the peptide nanotube-folic acid modified electrode lowered the electron transfer resulting in a decrease in the measured current. A detection limit of 250 human cervical cancer cells per mL was obtained. Control experiments confirmed that the peptide nanotube-folic acid electrode specifically recognized folate receptors. The modified electrode described here opens up new possibilities for future applications in early stage diagnoses of diseases where cells over-express folate receptors, such as in cancer or leishmaniasis disease.
Authors:
John J Castillo; Winnie E Svendsen; Noemi Rozlosnik; Patricia Escobar; Fernando Martínez; Jaime Castillo-León
Related Documents :
20363745 - Maturation of collagen ketoimine cross-links by an alternative mechanism to pyridinolin...
11068205 - Stability and networks of hydrogen bonds of the collagen triple helical structure: infl...
6321095 - Characterization of a large fragment from annelid cuticle collagen and its relationship...
8325895 - Characterization of an arginine 789 to cysteine substitution in alpha 1 (ii) collagen c...
20534455 - Diabetogenic t cells recognize insulin bound to iag7 in an unexpected, weakly binding r...
811255 - Modification of the automated sequence determination as applied to the sequence determi...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-13
Journal Detail:
Title:  The Analyst     Volume:  -     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Centro de Investigaciones en Catálisis, Universidad Industrial de Santander, Sede Guatiguará UIS Km 2 vía Refugio, Piedecuesta, Colombia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus.
Next Document:  Toward development of a surface-enhanced Raman scattering (SERS)-based cancer diagnostic immunoassay...