Document Detail


Detection of c-mos related products in the dogfish (Scyliorhinus canicula) testis.
MedLine Citation:
PMID:  7664974     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of the present paper was to do a comparative study to assess somatic versus germ cell localization of c-mos products in the testis. In mouse and amphibian oocytes, c-mos activity is necessary for meiotic maturation. Lack of c-mos expression has been reported in somatic cells of male and female gonads while transcripts have been found in germ cells of testis and ovary. Using a v-mos probe, we report here the detection of a c-mos related transcript (1.7 kb) in the dogfish Scyliorhinus canicula testis. Western blot analysis detects two proteins of 106 and 32 kDa. A specific immunostaining was exclusively localized in the interstitial tissue while the germinal compartment was completely negative. In conclusion, our results indicate for the first time the presence of c-mos products in an elasmobranch species and, moreover, their presence in somatic testicular cells rather than germ cells. Therefore, this finding in an ancient vertebrate indicates that c-mos activity does not have a direct universal role in the regulation of spermatogenesis.
Authors:
S Fasano; P Chieffi; S Minucci; K Le Guellec; B Jégou; R Pierantoni
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  109     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-10-10     Completed Date:  1995-10-10     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  127-32     Citation Subset:  IM    
Affiliation:
Dipartimento di Fisiologia Umana e Funzioni Biologiche Integrate F. Bottazzi, Facoltà di Medicina, Il Università di Napoli, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Dogfish / metabolism*
Male
Molecular Weight
Proto-Oncogene Proteins c-mos / analysis*,  genetics,  immunology,  physiology
RNA, Messenger / analysis
Sertoli Cells / chemistry*
Spermatocytes / chemistry
Spermatogenesis
Testis / chemistry,  cytology*
Chemical
Reg. No./Substance:
0/RNA, Messenger; EC 2.7.11.1/Proto-Oncogene Proteins c-mos

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