| Detection of bovine leukocyte antigen DRB3 alleles as candidate markers for clinical mastitis resistance in Holstein x Zebu. | |
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MedLine Citation:
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PMID: 18820165 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bovine leukocyte antigen DRB3 alleles from Holstein x Zebu crossbred dairy cows (n = 409) were analyzed using the PCR-RFLP technique. Exon II of DRB3 was amplified using locus-specific primers (HLO30/HLO32), followed by digestion with 3 restriction enzymes (RsaI, BstyI, and HaeIII). Forty alleles were found with frequency ranging from 0.005 to 0.139. The most frequently detected alleles of Holstein x Zebu were DRB3*16, *51, *23, *11, *8, and *1, accounting for 61.12% of the alleles in the population. Detection of candidate alleles for clinical mastitis occurrence was performed by logistic regression. It was found that percentage of Holstein fraction in crossbred cows had a nonsignificant effect (P > 0.05). However, parity had a significant effect on mastitis occurrence. In addition, DRB3*1 and *52 were the most associated with the occurrence of clinical mastitis, whereas *15, *51, and *22 were associated with resistance in crossbred populations. This is the first report of association of DRB3*15 and *51 with mastitis resistance. The association was validated by examining the candidate alleles in another commercial population. Highly susceptible (n = 43) and resistant (n = 42) groups of Holstein x Zebu cows were investigated. The result confirmed that DRB3*1 and *52 could be considered as susceptibility alleles, whereas *15, *51, and *22 could be considered as resistant alleles in Holstein x Zebu raised under tropical conditions. In addition, allele effects on 305-d milk production were estimated by BLUP. It was shown that most alleles associated with high clinical mastitis occurrence were related to increased milk yield. This study revealed that allele DRB3*10 had the greatest effect on increasing milk yield with moderate resistance to clinical mastitis, which could be used as a potential marker for selection in dairy genetic evaluation. |
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Authors:
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M Duangjinda; D Buayai; V Pattarajinda; Y Phasuk; S Katawatin; T Vongpralub; A Chaiyotvittayakul |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-26 |
Journal Detail:
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Title: Journal of animal science Volume: 87 ISSN: 1525-3163 ISO Abbreviation: J. Anim. Sci. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-26 Completed Date: 2009-04-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8003002 Medline TA: J Anim Sci Country: United States |
Other Details:
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Languages: eng Pagination: 469-76 Citation Subset: IM |
Affiliation:
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Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Thailand. monchai@kku.ac.th |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/EU586800; EU586801; EU586802; EU586803; EU586804; EU586805; EU586806; EU586807; EU586808 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Breeding* Cattle Female Gene Frequency Genetic Markers / genetics* Histocompatibility Antigens / genetics* Immunity, Innate / genetics* Lactation / genetics Male Mastitis, Bovine / diagnosis, genetics* Milk / secretion Molecular Sequence Data Polymorphism, Genetic Reproducibility of Results |
| Chemical | |
Reg. No./Substance:
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0/Genetic Markers; 0/Histocompatibility Antigens |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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