Document Detail

Detection of bovine herpesvirus 4 (BoHV-4) DNA in the cell fraction of milk of dairy cattle with history of BoHV-4 infection.
MedLine Citation:
PMID:  11101621     Owner:  NLM     Status:  MEDLINE    
We have demonstrated, by PCR and restriction enzyme analysis of the PCR product, the presence of bovine herpesvirus 4 (BoHV-4) DNA in the cell fraction of milk from dairy cattle with a history of BoHV-4 infection. We next evaluated the infectious nature of BoHV-4 DNA in those cells. Cocultivation of a BoHV-4-sensitive cell line with BoHV-4 DNA-positive milk cell samples produced cytopathic effects. The same result was obtained from frozen and thawed milk cell fraction coming from the cell milk fraction PCR-positive cows, ensuring that cells were killed and only infectious virus could be recovered after cocultivation with sensitive cells. This report shows that infectious BoHV-4 can be present in milk cells and that therefore nursing may be one of the transmission routes of BoHV-4.
G Donofrio; C F Flammini; F Scatozza; S Cavirani
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  38     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-01-03     Completed Date:  2001-01-03     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4668-71     Citation Subset:  IM    
Istituto di Malattie Infettive Profilassi e Polizia Veterinaria, Facoltà di Medicina Veterinaria, Università di Parma, 43100 Parma, Italy.
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MeSH Terms
Betaherpesvirinae / genetics,  isolation & purification*
Cattle Diseases / virology*
Cell Line
DNA, Viral / analysis*
Herpesviridae Infections / veterinary*
Milk / virology*
Reg. No./Substance:
0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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