Document Detail


Detection of biological macromolecules on a biochip dedicated to UV specific absorption.
MedLine Citation:
PMID:  18823766     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This work describes an ultraviolet biosensing technique based on specific molecular absorption detected with a previously developed spectrally selective aluminum gallium nitride (AlGaN) based detector. Light absorption signal of DNA and proteins, respectively at 260 nm and 280 nm, is used to image biochips. To allow detection of protein or DNA monolayers at the surface of a biochip, we develop contrast-enhancing multilayer substrates. We analyze them through models and experiments and validate the possibility of measuring absorptions of the order of 10(-3). These multilayer structures display a high reflectivity, and maximize the interaction of the electric field with the biological element at the chip surface. Optimization of the experimental absorption, which includes effects such as roughness of the biochip, spectral and angular resolution of the optics, illumination, etc., is carried out with an inorganic ultraviolet absorber (titanium dioxide) deposit. We obtained an induced absorption contrast enhanced by a factor of 4.0, conferring enough sensitivity to detect monolayers of DNA or proteins. Experimental results on an Escherichia coli histidine-tagged methionyl-tRNA synthetase protein before and after complexation with an anti-polyHis specific antibody validate our biosensing technique. This label-free optical method may be helpful in controlling biochip coatings, and subsequent biological coupling at the surface of a biochip.
Authors:
Kristelle Robin; Jean-Luc Reverchon; Laurent Mugherli; Michel Fromant; Pierre Plateau; Henri Benisty
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2008-08-27
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  24     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-02     Completed Date:  2009-05-26     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  1585-91     Citation Subset:  IM    
Affiliation:
Thales Research and Technology, Palaiseau Cedex, France. kristelle.robin@thalesgroup.com
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MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques / instrumentation*
DNA / analysis*
Equipment Design
Equipment Failure Analysis
Macromolecular Substances / analysis
Microchip Analytical Procedures / instrumentation*
Photometry / instrumentation*,  methods
Proteins / analysis*
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet / instrumentation*,  methods
Chemical
Reg. No./Substance:
0/Macromolecular Substances; 0/Proteins; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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