| Detection of biological macromolecules on a biochip dedicated to UV specific absorption. | |
| | |
MedLine Citation:
|
PMID: 18823766 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This work describes an ultraviolet biosensing technique based on specific molecular absorption detected with a previously developed spectrally selective aluminum gallium nitride (AlGaN) based detector. Light absorption signal of DNA and proteins, respectively at 260 nm and 280 nm, is used to image biochips. To allow detection of protein or DNA monolayers at the surface of a biochip, we develop contrast-enhancing multilayer substrates. We analyze them through models and experiments and validate the possibility of measuring absorptions of the order of 10(-3). These multilayer structures display a high reflectivity, and maximize the interaction of the electric field with the biological element at the chip surface. Optimization of the experimental absorption, which includes effects such as roughness of the biochip, spectral and angular resolution of the optics, illumination, etc., is carried out with an inorganic ultraviolet absorber (titanium dioxide) deposit. We obtained an induced absorption contrast enhanced by a factor of 4.0, conferring enough sensitivity to detect monolayers of DNA or proteins. Experimental results on an Escherichia coli histidine-tagged methionyl-tRNA synthetase protein before and after complexation with an anti-polyHis specific antibody validate our biosensing technique. This label-free optical method may be helpful in controlling biochip coatings, and subsequent biological coupling at the surface of a biochip. |
| | |
Authors:
|
Kristelle Robin; Jean-Luc Reverchon; Laurent Mugherli; Michel Fromant; Pierre Plateau; Henri Benisty |
Related Documents
:
|
23258296 - Protein homeostasis: live long, won't prosper. 19441486 - A gold nanoparticle-based microfluidic protein chip for tumor markers. 23362196 - Recent advances in the electrochemistry and spectroelectrochemistry of membrane proteins. 179596 - Cell surface and metabolic labelling of the proteins of normal and transformed chicken ... 8790826 - Characterization of latex allergens and correlation of serum ige/igg antibody ratio wit... 2553076 - Cytochrome c methylation. |
Publication Detail:
|
Type: Evaluation Studies; Journal Article Date: 2008-08-27 |
Journal Detail:
|
Title: Biosensors & bioelectronics Volume: 24 ISSN: 1873-4235 ISO Abbreviation: Biosens Bioelectron Publication Date: 2009 Feb |
Date Detail:
|
Created Date: 2009-02-02 Completed Date: 2009-05-26 Revised Date: 2009-07-14 |
Medline Journal Info:
|
Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
|
Languages: eng Pagination: 1585-91 Citation Subset: IM |
Affiliation:
|
Thales Research and Technology, Palaiseau Cedex, France. kristelle.robin@thalesgroup.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biosensing Techniques
/
instrumentation* DNA / analysis* Equipment Design Equipment Failure Analysis Macromolecular Substances / analysis Microchip Analytical Procedures / instrumentation* Photometry / instrumentation*, methods Proteins / analysis* Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet / instrumentation*, methods |
| Chemical | |
Reg. No./Substance:
|
0/Macromolecular Substances; 0/Proteins; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Immunohistochemical study on the distribution of insulin-like growth factor-binding protein 4 in the...
Next Document: The role of H2O2 outer diffusion on the performance of implantable glucose sensors.