| Detection of benzodiazepine intake in therapeutic doses by immunoanalysis of urine: two techniques evaluated and modified for improved performance. | |
| | |
MedLine Citation:
|
PMID: 1541010 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We evaluated the EMIT (enzyme-multiplied immuno technique) and FPIA (fluorescence polarization immunoassay) urine screening systems for detection of benzodiazepine intake. Healthy male volunteers were given single oral therapeutic doses of alprazolam (2 mg), chlordiazepoxide (25 mg), flunitrazepam (1 mg), lorazepam (3.75 mg), nitrazepam (5 mg), and triazolam (0.25 mg), after which urine was collected for the next 32 h. The EMIT method failed to detect the intake of flunitrazepam, lorazepam, and nitrazepam. FPIA did not detect the intake of chlordiazepoxide, flunitrazepam, lorazepam, nitrazepam, and triazolam. Modification of the EMIT method to include enzymatic hydrolysis did not significantly alter the results obtained with this method. A modification of the FPIA method to include enzymatic hydrolysis and a lower cutoff value improved the results considerably, so that we reliably detected all studied substances but flunitrazepam. We conclude that (a) both EMIT and FPIA techniques, when used as intended by the manufacturers, are unreliable for the detection of intake of therapeutic doses of these benzodiazepines, and (b) the described modification of the FPIA should provide a much improved tool for detection of benzodiazepine intake. |
| | |
Authors:
|
O Beck; P Lafolie; P Hjemdahl; S Borg; G Odelius; P Wirbing |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Clinical chemistry Volume: 38 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1992 Feb |
Date Detail:
|
Created Date: 1992-04-03 Completed Date: 1992-04-03 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 271-5 Citation Subset: IM |
Affiliation:
|
Department of Clinical Pharmacology, Karolinska Hospital, Stockholm, Sweden. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Alprazolam / pharmacokinetics, urine Benzodiazepines / urine* Chlordiazepoxide / pharmacokinetics, urine False Negative Reactions Flunitrazepam / pharmacokinetics, urine Fluorescence Polarization Immunoassay / standards* Humans Immunoenzyme Techniques / standards* Lorazepam / pharmacokinetics, urine Male Middle Aged Nitrazepam / pharmacokinetics, urine Triazolam / pharmacokinetics, urine |
| Chemical | |
Reg. No./Substance:
|
12794-10-4/Benzodiazepines; 146-22-5/Nitrazepam; 1622-62-4/Flunitrazepam; 28911-01-5/Triazolam; 28981-97-7/Alprazolam; 58-25-3/Chlordiazepoxide; 846-49-1/Lorazepam |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Improved detection of ischemia-induced increases in coronary sinus adenosine in patients with corona...
Next Document: When do gut flora in the newborn produce 3-phenylpropionic acid? Implications for early diagnosis of...