| Detection of autoantibodies to survivin in cervical mucus from patients with human papillomavirus-associated cervical cancer and precursor lesions. | |
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MedLine Citation:
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PMID: 17364499 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: To investigate the prevalence of mucosal autoantibodies to survivin in patients with human papillomavirus (HPV)-associated cervical cancer and precursor lesions. METHODS: Cervical mucus from 117 HPV-associated cervical cancer, 102 high-grade squamous intraepithelial lesion (HSIL), 107 low-grade SIL (LSIL), and 80 normal controls were tested by ELISA using either full length recombinant survivin or survivin-derived peptides. Survivin expression in cervical tissue biopsies was studied by Western Blotting. RESULTS: Cervical mucus from 33 cervical cancer (28.2%), 17 HSIL (16.6%), and 8 LSIL (7.4%) patients reacted with recombinant survivin. The IgA-class antibody response was significantly higher than that observed in the normal controls. The level of mucosal anti-survivin response was associated to the level and intensity of survivin expression in the different lesions. Finally, reactivity against a survivin Nt-derived peptide was found more frequently than reactivity against a Ct-derived peptide. CONCLUSIONS: IgA-class autoantibodies against survivin are present in a substantial proportion of cervical mucus from patients with HPV-associated cervical cancer, and precursor lesions. Mucosal anti-survivin response is positively associated with the level of survivin expression and the grade of cervical lesion. |
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Authors:
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Lizbeth Gonzalez Jimenez; Maria Cristina Aguilar; Olga L Monroy; Fernando Cruz-Talonia; Rosa M Cruz; Carlos Huitron; Leticia Rocha-Zavaleta |
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Publication Detail:
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Type: Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Autoimmunity Volume: 40 ISSN: 0891-6934 ISO Abbreviation: Autoimmunity Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-03-16 Completed Date: 2007-06-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8900070 Medline TA: Autoimmunity Country: England |
Other Details:
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Languages: eng Pagination: 66-72 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology and Biotechnology, Institute of Biomedical Research, National University of Mexico, Circuito Escolar s/n, Ciudad Universitaria, Mexico City, CP 04510, Mexico. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Autoantibodies
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biosynthesis*,
immunology Cervical Intraepithelial Neoplasia / immunology*, virology Cervix Mucus / immunology* Enzyme-Linked Immunosorbent Assay / methods Female Humans Immunoglobulin A / immunology Microtubule-Associated Proteins / biosynthesis, immunology* Neoplasm Proteins / biosynthesis, immunology* Papillomaviridae / immunology* Papillomavirus Infections / complications, immunology* Prospective Studies Uterine Cervical Neoplasms / immunology*, pathology, virology |
| Chemical | |
Reg. No./Substance:
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0/Autoantibodies; 0/BIRC5 protein, human; 0/Immunoglobulin A; 0/Microtubule-Associated Proteins; 0/Neoplasm Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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