Document Detail


Detection of auto-antibodies against cytochrome P4502E1 (CYP2E1) in chronic hepatitis C.
MedLine Citation:
PMID:  17196701     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIMS: Chronic hepatitis C (CHC) is often associated with auto-immune reactions. In the light of the role of alcohol in promoting CHC progression, we have investigated the possible presence of auto-reactivity against the ethanol-inducible cytochrome P4502E1 (CYP2E1) in CHC patients with and without alcohol consumption. METHODS: The IgG reactivity against recombinant human CYP2E1 was evaluated by solid-phase immunoassays in 102 CHC patients with different alcohol consumption and 59 HCV-free controls. RESULTS: Auto-antibodies against CYP2E1 were significantly (p<0.0001) increased in CHC patients as compared to controls. Anti-CYP2E1 IgG above the 97th percentile in the controls were evident in 41 (40%) CHC patients. Competition experiments revealed that CYP2E1 recognition was not due to the cross-reactivity with CYP2D6. The detection of anti-CYP2E1 IgG was unrelated to alcohol consumption and no difference in gender, age, aminotransferase levels and virus genotype was evident among the patients with or without anti-CYP2E1 auto-antibodies. However, anti-CYP2E1 auto-reactivity was significantly (p=0.025) associated with the severity of periportal/periseptal interface hepatitis. Moreover, confocal microscopy demonstrated that anti-CYP2E1 IgG associated with CHC recognized CYP2E1 exposed on the outer side of hepatocyte plasma membranes. CONCLUSIONS: HCV infection favours the breaking of self-tolerance against CYP2E1 that might contribute to hepatocyte injury.
Authors:
Matteo Vidali; Giuseppa Occhino; Alessandra Ivaldi; Roberto Serino; Stefania Moia; Elisa Alchera; Rita Carini; Cristina Rigamonti; Massimo Sartori; Emanuele Albano
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-06
Journal Detail:
Title:  Journal of hepatology     Volume:  46     ISSN:  0168-8278     ISO Abbreviation:  J. Hepatol.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-19     Completed Date:  2007-06-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8503886     Medline TA:  J Hepatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  605-12     Citation Subset:  IM    
Affiliation:
Department of Medical Sciences, University Amedeo Avogadro of East Piedmont and Interdepartmental Research Centre for Autoimmune Diseases (IRCAD), Novara, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Alcohol Drinking
Animals
Autoantibodies / blood*
Cell Membrane / metabolism
Cytochrome P-450 CYP2E1 / immunology*,  metabolism
Female
Hepatitis C, Chronic / immunology*,  metabolism,  physiopathology
Hepatocytes / metabolism
Humans
Immunoglobulin G / immunology
Male
Microscopy, Confocal
Middle Aged
Rats
Rats, Sprague-Dawley
Severity of Illness Index
Tissue Distribution
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Immunoglobulin G; EC 1.14.14.1/Cytochrome P-450 CYP2E1

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