| Detection of aquareovirus RNA in fish tissues by nucleic acid hybridization with a cloned cDNA probe. | |
| | |
MedLine Citation:
|
PMID: 8391024 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A nucleic acid hybridization assay was developed to rapidly detect small quantities of aquareovirus RNAs in infected cells and organs. Cloned cDNA copies were synthesized from the genomic RNA of the SBR strain of aquareovirus. By using cloned cDNA probes, aquareovirus RNAs were detected in spleen and kidney tissues of experimentally infected fish. |
| | |
Authors:
|
K Subramanian; B Lupiani; F M Hetrick; S K Samal |
Related Documents
:
|
16116034 - Conversions of formaldehyde-modified 2'-deoxyadenosine 5'-monophosphate in conditions m... 7248264 - Affinities of nucleic acid bases for solvent water. 9759484 - Modified oligonucleotides: synthesis and strategy for users. 5338564 - The enzymatic phosphorylation of nucleic acids and its application to end-group analysis. 6594094 - Long-term (8 days) effects of exposure to low concentrations of fluoride on enamel form... 19585824 - A postmarketing surveillance study of dexrabeprazole in the treatment of acid peptic di... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Journal of clinical microbiology Volume: 31 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 1993 Jun |
Date Detail:
|
Created Date: 1993-07-28 Completed Date: 1993-07-28 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1612-4 Citation Subset: IM |
Affiliation:
|
College of Veterinary Medicine, University of Maryland, College Park 20742. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cloning, Molecular DNA Probes Fish Diseases / diagnosis, microbiology Fishes / microbiology* Kidney / microbiology Nucleic Acid Hybridization RNA, Viral / genetics, isolation & purification* Reoviridae / classification, genetics, isolation & purification* Reoviridae Infections / diagnosis, microbiology, veterinary Spleen / microbiology Trout / microbiology |
| Chemical | |
Reg. No./Substance:
|
0/DNA Probes; 0/RNA, Viral |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Prevalence of human herpesvirus 6 variant A and B infections in bone marrow transplant recipients as...
Next Document: Subclass-specific serum antibody responses to recombinant Norwalk virus capsid antigen (rNV) in adul...