| Detection of apoptosis in cardiovascular diseases. | |
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MedLine Citation:
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PMID: 16010024 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The past decade has seen a surge in research devoted to understanding the role of cell death in the pathogenesis of various forms of cardiovascular disease. In particular, apoptosis has received much attention owing to the tightly regulated biochemical nature of this form of cell death and the realization of potential therapeutic opportunities. The current chapter describes a few of the more widely used protocols for detecting and quantifying apoptosis in cardiovascular tissues. Specifically, this chapter describes terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining for DNA fragmentation, Hoechst staining for chromatin condensation, annexin V labeling of phosphatidylserine externalization, and Western blot and immunoflorescence detection of caspase cleavage and activation, respectively. |
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Authors:
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Arwen L Hunter; Jonathan C Choy; David J Granville |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Methods in molecular medicine Volume: 112 ISSN: 1543-1894 ISO Abbreviation: Methods Mol. Med. Publication Date: 2005 |
Date Detail:
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Created Date: 2005-07-12 Completed Date: 2005-08-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101123138 Medline TA: Methods Mol Med Country: United States |
Other Details:
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Languages: eng Pagination: 277-89 Citation Subset: IM |
Affiliation:
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Department of Pathology and Laboratory Medicine, St. Paul's Hospital, University of British Columbia, Vancouver, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Annexin A5 / metabolism Apoptosis* Cardiovascular Diseases / metabolism, pathology, physiopathology* Caspases / analysis Cell Death Cells, Cultured DNA Fragmentation Endothelium, Vascular / cytology, metabolism Humans In Situ Nick-End Labeling Mitochondria, Heart / metabolism Muscle, Smooth, Vascular Myocytes, Cardiac / cytology, metabolism Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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0/Annexin A5; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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