Document Detail


Detection of apoptosis in cardiovascular diseases.
MedLine Citation:
PMID:  16010024     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The past decade has seen a surge in research devoted to understanding the role of cell death in the pathogenesis of various forms of cardiovascular disease. In particular, apoptosis has received much attention owing to the tightly regulated biochemical nature of this form of cell death and the realization of potential therapeutic opportunities. The current chapter describes a few of the more widely used protocols for detecting and quantifying apoptosis in cardiovascular tissues. Specifically, this chapter describes terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining for DNA fragmentation, Hoechst staining for chromatin condensation, annexin V labeling of phosphatidylserine externalization, and Western blot and immunoflorescence detection of caspase cleavage and activation, respectively.
Authors:
Arwen L Hunter; Jonathan C Choy; David J Granville
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Methods in molecular medicine     Volume:  112     ISSN:  1543-1894     ISO Abbreviation:  Methods Mol. Med.     Publication Date:  2005  
Date Detail:
Created Date:  2005-07-12     Completed Date:  2005-08-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101123138     Medline TA:  Methods Mol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  277-89     Citation Subset:  IM    
Affiliation:
Department of Pathology and Laboratory Medicine, St. Paul's Hospital, University of British Columbia, Vancouver, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Annexin A5 / metabolism
Apoptosis*
Cardiovascular Diseases / metabolism,  pathology,  physiopathology*
Caspases / analysis
Cell Death
Cells, Cultured
DNA Fragmentation
Endothelium, Vascular / cytology,  metabolism
Humans
In Situ Nick-End Labeling
Mitochondria, Heart / metabolism
Muscle, Smooth, Vascular
Myocytes, Cardiac / cytology,  metabolism
Staining and Labeling
Chemical
Reg. No./Substance:
0/Annexin A5; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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