Document Detail

Detection of antizona pellucida antibodies in the sera from premature ovarian failure patients by a highly specific test.
MedLine Citation:
PMID:  17349637     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To develop a highly specific test for the detection of antizona pellucida (ZP) antibodies in the sera from premature ovarian failure (POF) patients. DESIGN: Laboratory study. SETTING: University hospital. PATIENT(S): Twenty-seven idiopathic POF patients, 30 control women, and 30 healthy males. INTERVENTION(S): Anti-ZP antibodies were detected by the microdot assay using a very small amount of human ZP or porcine ZP. The effect of anti-ZP antibodies on sperm-ZP binding was examined by hemizona assay. MAIN OUTCOME MEASURE(S): Results from the microdot assay and hemizona assay. RESULT(S): By the microdot assay using human ZP, the sera from POF patients reacted significantly stronger than those of control women and healthy males. However, no obvious difference could be found by the same assay using porcine ZP among these three groups. Anti-ZP antibodies against sera from some POF patients showed significant blocking effects on sperm-ZP binding assessed by hemizona assay. Anti-ZP antibodies were detected in 7 of 27 POF patients, while none were detected in control women and healthy males. CONCLUSION(S): Some idiopathic POF patients have anti-ZP antibodies in their sera, which were detected with high specificity by a newly developed microdot assay using a very small amount of human ZP.
Satoru Takamizawa; Hiroaki Shibahara; Tamaho Shibayama; Mitsuaki Suzuki
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Publication Detail:
Type:  Journal Article     Date:  2007-03-08
Journal Detail:
Title:  Fertility and sterility     Volume:  88     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-08     Completed Date:  2007-11-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  925-32     Citation Subset:  IM    
Department of Obstetrics and Gynecology, School of Medicine, Jichi Medical University, Tochigi, Japan.
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MeSH Terms
Autoantibodies / analysis*
Immunoblotting / methods*
Ovarian Failure, Premature / immunology*
Sperm-Ovum Interactions / immunology
Swine / immunology
Zona Pellucida / immunology*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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