Document Detail


Detection of aneuploidy in interphase nuclei from non-small cell lung carcinomas by fluorescence in situ hybridization using chromosome-specific repetitive DNA probes.
MedLine Citation:
PMID:  8697416     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interphase fluorescence in situ hybridization (FISH) is particularly useful for detecting chromosome changes in tumors exhibiting a low mitotic index, as is the case in many human non-small cell lung carcinomas (NSCLCs). A panel of centromeric DNA probes specific for the autosomes 6, 7, 8, 9, 12, 17, and 18 was used to analyze 17 primary NSCLCs. Evidence for aneuploidy was obtained in all specimens. Gain of part or all of chromosome 7 was especially prominent, occurring in a large population of cells in each of 14 tumors (82%). Extra centromeric copies of chromosomes 6, 12, and 17 were also common, being observed in 9 to 11 cases each. Gain of chromosome 9 was infrequent (three tumors). In two cases, most of the nuclei had only a single chromosome 9 fluorescent signal. Karyotypic findings were available for six cases and were generally consistent with the FISH data. Both methods revealed considerable heterogeneity within individual tumors. NSCLC specimens from 26 males were assayed with a Y-specific centromeric sequence; loss of the Y was observed in 13 cases (50%). These investigations demonstrate the feasibility of interphase FISH for the successful analysis of numerical chromosome changes in NSCLCs.
Authors:
T Taguchi; J Y Zhou; M Feder; S Litwin; A J Klein-Szanto; J R Testa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer genetics and cytogenetics     Volume:  89     ISSN:  0165-4608     ISO Abbreviation:  Cancer Genet. Cytogenet.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-08-30     Completed Date:  1996-08-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7909240     Medline TA:  Cancer Genet Cytogenet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  120-5     Citation Subset:  IM    
Affiliation:
Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.
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MeSH Terms
Descriptor/Qualifier:
Aneuploidy*
Carcinoma, Non-Small-Cell Lung / genetics*
Cell Nucleus / ultrastructure
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 6
Chromosomes, Human, Pair 7
DNA Probes*
Humans
In Situ Hybridization, Fluorescence*
Interphase
Lung Neoplasms / genetics*
Repetitive Sequences, Nucleic Acid*
Grant Support
ID/Acronym/Agency:
CA-06927/CA/NCI NIH HHS; CA-45745/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/DNA Probes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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