Document Detail

Detection of anaerobic metabolites of saturated and aromatic hydrocarbons in petroleum-contaminated aquifers.
MedLine Citation:
PMID:  12322748     Owner:  NLM     Status:  MEDLINE    
Recent investigations have demonstrated that several classes of petroleum hydrocarbons are susceptible to anaerobic decay, including alkanes and mono- and polycyclic aromatic compounds. In previous work, benzylsuccinates were shown to be useful indicators of in situ anaerobic alkylbenzene metabolism. In the present study, we sought to determine whether metabolites of alkanes and naphthalenes could similarly be used as indicators of the intrinsic decomposition of these compounds in petroleum-contaminated aquifers. Such metabolites include succinate derivatives of n-alkanes, cyclic alkanes, and alkylaromatic hydrocarbons as well as naphthoic acids. Using gas chromatography-mass spectrometry (GC-MS), we analyzed trimethylsilyl-derivatized organic extracts from six hydrocarbon-contaminated groundwaters for MS fragment ions indicative of such anaerobic metabolites. Geochemical indicators in these aquifers suggested the prevalence of anaerobic processes. In the groundwaters of the contaminated sites, we found compounds whose MS profiles suggested that they were indeed alkylsuccinic acids, ranging from C3 to C11 succinates. Propyl-, hexyl-, octyl-, and decylsuccinic acids were positively identified in the groundwaters by GC-MS matches with chemical or biologically produced standards. In two of the aquifers, we also detected components whose MS profiles matched with authentic standards of naphthoic acids and tetrahydronaphthoic acids. Metabolites were detected in nanomolar concentrations. The finding of these putative anaerobic metabolites of alkanes and naphthalenes signifies the in situ biodegradation of these hydrocarbons and attests to their value as indicators of intrinsic bioremediation.
Lisa M Gieg; Joseph M Suflita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Environmental science & technology     Volume:  36     ISSN:  0013-936X     ISO Abbreviation:  Environ. Sci. Technol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-26     Completed Date:  2003-02-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0213155     Medline TA:  Environ Sci Technol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3755-62     Citation Subset:  IM    
Department of Botany and Microbiology and Institute for Energy and the Environment, University of Oklahoma, Norman 73019, USA.
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MeSH Terms
Biodegradation, Environmental
Fresh Water / chemistry
Gas Chromatography-Mass Spectrometry
Hydrocarbons, Aromatic / analysis*,  chemistry
Petroleum / analysis*
Water Pollutants, Chemical / analysis*
Reg. No./Substance:
0/Hydrocarbons, Aromatic; 0/Petroleum; 0/Water Pollutants, Chemical

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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