Document Detail


Detection of amino acid and peptide transmitters in physiologically identified brainstem cardiorespiratory neurons.
MedLine Citation:
PMID:  15331039     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Most of the CNS neurons that regulate circulation and respiration reside in regions of the brain characterized by extreme cellular heterogeneity (nucleus of the solitary tract, reticular formation, parabrachial nuclei, periaqueductal gray matter, hypothalamus, etc.). The chemical neuroanatomy of these regions is correspondingly complex and teasing out specific circuits in their midst remains a problem that is usually very difficult if not impossible to solve by conventional tract-tracing methods, Fos methodology or electrophysiology in slices. In addition, identifying the type of amino acid or peptide transmitter used by electrophysiologically recorded neurons has been until recently an especially difficult task either for lack of a specific marker or because such markers (many peptides for example) are exported to synaptic terminals and thus undetectable in neuronal cell bodies. In this review, we describe a general purpose method that solves many of these problems. The approach combines juxtacellular labeling in vivo with the histological identification of mRNAs that provide definitive neurochemical phenotypic identification (e.g. vesicular glutamate transporter 1 or 2, glutamic acid decarboxylase). The results obtained with this method are discussed in the general context of amino acid transmission in brainstem cardiorespiratory pathways. The presence of markers of amino acid transmission in specific aminergic pre-sympathetic neurons is especially emphasized as is the extensive co-localization of markers of GABAergic and glycinergic transmission in the brainstem reticular formation.
Authors:
Patrice G Guyenet; Ruth L Stornetta; Matthew C Weston; Travis McQuiston; Johnny R Simmons
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Autonomic neuroscience : basic & clinical     Volume:  114     ISSN:  1566-0702     ISO Abbreviation:  Auton Neurosci     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-08-27     Completed Date:  2004-11-29     Revised Date:  2009-08-12    
Medline Journal Info:
Nlm Unique ID:  100909359     Medline TA:  Auton Neurosci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1-10     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, University of Virginia, Charlottesville, VA 22908, United States.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Transport Systems, Neutral / genetics,  metabolism
Amino Acids / analysis*,  chemistry
Animals
Brain Stem / cytology*,  metabolism
Glutamate Decarboxylase / genetics,  metabolism
Glycine Plasma Membrane Transport Proteins
Membrane Transport Proteins*
Neural Networks (Computer)
Neurons / chemistry*,  classification,  metabolism
Peptides / analysis*,  chemistry
Respiration*
Synaptic Transmission / genetics,  physiology
Vesicular Glutamate Transport Protein 2
Vesicular Transport Proteins / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
HL 074011/HL/NHLBI NIH HHS; HL 28785/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acid Transport Systems, Neutral; 0/Amino Acids; 0/Glycine Plasma Membrane Transport Proteins; 0/Membrane Transport Proteins; 0/Peptides; 0/Vesicular Glutamate Transport Protein 2; 0/Vesicular Transport Proteins; EC 4.1.1.15/Glutamate Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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