| Detection of amino acid and peptide transmitters in physiologically identified brainstem cardiorespiratory neurons. | |
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MedLine Citation:
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PMID: 15331039 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Most of the CNS neurons that regulate circulation and respiration reside in regions of the brain characterized by extreme cellular heterogeneity (nucleus of the solitary tract, reticular formation, parabrachial nuclei, periaqueductal gray matter, hypothalamus, etc.). The chemical neuroanatomy of these regions is correspondingly complex and teasing out specific circuits in their midst remains a problem that is usually very difficult if not impossible to solve by conventional tract-tracing methods, Fos methodology or electrophysiology in slices. In addition, identifying the type of amino acid or peptide transmitter used by electrophysiologically recorded neurons has been until recently an especially difficult task either for lack of a specific marker or because such markers (many peptides for example) are exported to synaptic terminals and thus undetectable in neuronal cell bodies. In this review, we describe a general purpose method that solves many of these problems. The approach combines juxtacellular labeling in vivo with the histological identification of mRNAs that provide definitive neurochemical phenotypic identification (e.g. vesicular glutamate transporter 1 or 2, glutamic acid decarboxylase). The results obtained with this method are discussed in the general context of amino acid transmission in brainstem cardiorespiratory pathways. The presence of markers of amino acid transmission in specific aminergic pre-sympathetic neurons is especially emphasized as is the extensive co-localization of markers of GABAergic and glycinergic transmission in the brainstem reticular formation. |
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Authors:
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Patrice G Guyenet; Ruth L Stornetta; Matthew C Weston; Travis McQuiston; Johnny R Simmons |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S.; Review |
Journal Detail:
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Title: Autonomic neuroscience : basic & clinical Volume: 114 ISSN: 1566-0702 ISO Abbreviation: Auton Neurosci Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-08-27 Completed Date: 2004-11-29 Revised Date: 2009-08-12 |
Medline Journal Info:
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Nlm Unique ID: 100909359 Medline TA: Auton Neurosci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-10 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, University of Virginia, Charlottesville, VA 22908, United States. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Transport Systems, Neutral
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genetics,
metabolism Amino Acids / analysis*, chemistry Animals Brain Stem / cytology*, metabolism Glutamate Decarboxylase / genetics, metabolism Glycine Plasma Membrane Transport Proteins Membrane Transport Proteins* Neural Networks (Computer) Neurons / chemistry*, classification, metabolism Peptides / analysis*, chemistry Respiration* Synaptic Transmission / genetics, physiology Vesicular Glutamate Transport Protein 2 Vesicular Transport Proteins / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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HL 074011/HL/NHLBI NIH HHS; HL 28785/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acid Transport Systems, Neutral; 0/Amino Acids; 0/Glycine Plasma Membrane Transport Proteins; 0/Membrane Transport Proteins; 0/Peptides; 0/Vesicular Glutamate Transport Protein 2; 0/Vesicular Transport Proteins; EC 4.1.1.15/Glutamate Decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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