Document Detail

Detection of active oxygen in rat hepatocyte suspensions with the chemiluminigenic probe lucigenin.
MedLine Citation:
PMID:  3022723     Owner:  NLM     Status:  MEDLINE    
The chemiluminigenic probe lucigenin has been employed to detect the production of active oxygen species in suspensions of intact rat hepatocytes. Light emission from lucigenin arises from oxygenation by superoxide anion; hydrogen peroxide or a species derived from it may contribute to the reaction. The inhibitory action of antioxidants on the availability of active oxygen species produced by hepatocytes was tested. Propyl gallate was the most potent inhibitor, butylated hydroxyanisole and butylated hydroxytoluene were less active. The latter compounds cause an alteration of the cell membrane at high concentrations.
R Kahl; A Weimann; A G Hildebrandt
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  140     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1986 Oct 
Date Detail:
Created Date:  1986-12-04     Completed Date:  1986-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  468-75     Citation Subset:  IM    
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MeSH Terms
Acridines / diagnostic use*
Antioxidants / pharmacology
Hydrogen Peroxide / analysis
Hydroxides / analysis
Hydroxyl Radical
Liver / analysis*
Luminescent Measurements
Oxygen / analysis*
Rats, Inbred Strains
Superoxides / analysis
Reg. No./Substance:
0/Acridines; 0/Antioxidants; 0/Hydroxides; 0/Suspensions; 11062-77-4/Superoxides; 2315-97-1/10,10'-dimethyl-9,9'-biacridinium; 3352-57-6/Hydroxyl Radical; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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