| Detection of active oxygen in rat hepatocyte suspensions with the chemiluminigenic probe lucigenin. | |
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MedLine Citation:
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PMID: 3022723 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The chemiluminigenic probe lucigenin has been employed to detect the production of active oxygen species in suspensions of intact rat hepatocytes. Light emission from lucigenin arises from oxygenation by superoxide anion; hydrogen peroxide or a species derived from it may contribute to the reaction. The inhibitory action of antioxidants on the availability of active oxygen species produced by hepatocytes was tested. Propyl gallate was the most potent inhibitor, butylated hydroxyanisole and butylated hydroxytoluene were less active. The latter compounds cause an alteration of the cell membrane at high concentrations. |
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Authors:
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R Kahl; A Weimann; A G Hildebrandt |
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2199343 - Mechanisms for regulating oxygen toxicity in phytophagous insects. 19482633 - Detection of superoxide in cells, tissues and whole organisms. 19423823 - An experimental design method leading to chemical turing patterns. |
Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 140 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1986 Oct |
Date Detail:
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Created Date: 1986-12-04 Completed Date: 1986-12-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 468-75 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acridines
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diagnostic use* Animals Antioxidants / pharmacology Hydrogen Peroxide / analysis Hydroxides / analysis Hydroxyl Radical Liver / analysis* Luminescent Measurements Male Oxygen / analysis* Rats Rats, Inbred Strains Superoxides / analysis Suspensions |
| Chemical | |
Reg. No./Substance:
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0/Acridines; 0/Antioxidants; 0/Hydroxides; 0/Suspensions; 11062-77-4/Superoxides; 2315-97-1/10,10'-dimethyl-9,9'-biacridinium; 3352-57-6/Hydroxyl Radical; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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