Document Detail


Detection of abnormalities in B-cell differentiation pattern is a useful tool to predict relapse in precursor-B-ALL.
MedLine Citation:
PMID:  10192428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Immunophenotypic investigation of minimal residual disease (MRD) has traditionally been based on the investigation of phenotypic aberrants at diagnosis to be used later as a target for MRD detection. This approach has several shortcomings (it is only applicable to patients with aberrant phenotypes, requires a diagnostic sample, and is patient-specific) and therefore a search for simpler alternatives is warranted. The present study is based on the hypothesis that in precursor-B-ALL patients the persistence of residual leukaemic cells may induce abnormalities in the precursor-B-cell compartment in bone marrow (BM) and these could be used as a criteria to predict relapse. These abnormalities may include: (1) the presence of an increase in the frequencies of immature B cells (CD34+/CD19+ or CD20-/CD19+) or (2) the existence of an altered B-cell differentiation pathway due to a blockade or to the presence of B cells outside the normal pathway. A total of 180 BM samples from 45 consecutive precursor-B-ALL patients who achieved morphological complete remission (CR) were analysed by multiparametric flow cytometry. Our results show that a significant increase in immature B-cell subsets or an altered B-cell differentiation predicts a high relapse rate (P<0.01) and a shorter disease-free survival (P<0.01). Moreover, abnormalities in either of these two criteria detected at specific time points during follow-up (end of induction, maintenance, or after treatment) were associated with a significantly shorter disease-free survival (P<0.01). In summary, the investigation of abnormalities in B-cell differentiation is a relatively simple and cheap approach for predicting relapse in precursor-B-ALL patients.
Authors:
J Ciudad; J F San Miguel; M C López-Berges; M A García Marcos; M González; L Vázquez; M C del Cañizo; A López; J J Van Dongen; A Orfao
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  British journal of haematology     Volume:  104     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-06-18     Completed Date:  1999-06-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  695-705     Citation Subset:  IM    
Affiliation:
Servicio General de Citometría, Universidad de Salamanca, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Antigens, CD / metabolism
Cell Transformation, Neoplastic
Child
Child, Preschool
Flow Cytometry
Fluorescent Antibody Technique
Follow-Up Studies
Humans
Infant
Middle Aged
Neoplasm, Residual / pathology
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / pathology*
Recurrence
Chemical
Reg. No./Substance:
0/Antigens, CD

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