Document Detail

Detection of ZAP-70 in patients with chronic lymphocytic leukemia.
MedLine Citation:
PMID:  19145677     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To determine the presence and prognostic significance of ZAP-70 in patients with B chronic lymphocytic leukemia (B-CLL). Expression of ZAP-70 in B-CLL is a continuum ranging from absent to high, affecting the prognostic reliability of this marker. PATIENTS AND METHODS: 32 patients with B-CLL treated from March 2006 to February 2007 were prospectively studied. Patients were stratified in two groups: those diagnosed with B-CLL within 18 months and a retrospective group diagnosed before 18 months since the beginning of this study. Patients were predominantly males (78.1%), over 60 years old (81.25%). ZAP-70 was detected on EPICX-XL flow cytometer with System II software. Directly conjugated antibodies CD19 PE, ZAP-70 FITC (clone 1E7.2) and CD2 APC were used. Leuoperm was used for permeabilization. All samples with over 20% ZAP-70/CD19 double positive B lymphocytes were considered positive. RESULTS: ZAP-70 was absent in a group of 22 patients with B-CLL diagnosed over 18 months from the beginning of this study, while 2 out of 10 (20%) patients from the group of newly diagnosed patients were ZAP-70(+). During follow up both ZAP-70(+) cases showed worsening of their clinical status resulting to death in one patient. In borderline cases with ZAP-70(+) ranging from 10.9-19.9% there was no correlation of the level of ZAP-70 expression with disease activity or clinical scoring systems. CONCLUSION: Very high percentage of ZAP-70(+) cells is a sign of poor prognosis in B-CLL. Borderline expression of ZAP-70, although more frequent, could not be successfully assigned into a risk group.
G Marjanovic; I Stanojevic; Lj Jankovic-Velickovic; W Hubl; V Marjanovic
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of B.U.ON. : official journal of the Balkan Union of Oncology     Volume:  13     ISSN:  1107-0625     ISO Abbreviation:  J BUON     Publication Date:    2008 Oct-Dec
Date Detail:
Created Date:  2009-01-15     Completed Date:  2009-03-19     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100883428     Medline TA:  J BUON     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  543-6     Citation Subset:  IM    
Clinic of Hematology, Clinical Centre Nis, Serbia.
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MeSH Terms
Flow Cytometry
Leukemia, Lymphocytic, Chronic, B-Cell / blood*,  mortality
Middle Aged
Prospective Studies
ZAP-70 Protein-Tyrosine Kinase / blood*
Reg. No./Substance:
EC Protein-Tyrosine Kinase

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