| Detection of ZAP-70 in patients with chronic lymphocytic leukemia. | |
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MedLine Citation:
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PMID: 19145677 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To determine the presence and prognostic significance of ZAP-70 in patients with B chronic lymphocytic leukemia (B-CLL). Expression of ZAP-70 in B-CLL is a continuum ranging from absent to high, affecting the prognostic reliability of this marker. PATIENTS AND METHODS: 32 patients with B-CLL treated from March 2006 to February 2007 were prospectively studied. Patients were stratified in two groups: those diagnosed with B-CLL within 18 months and a retrospective group diagnosed before 18 months since the beginning of this study. Patients were predominantly males (78.1%), over 60 years old (81.25%). ZAP-70 was detected on EPICX-XL flow cytometer with System II software. Directly conjugated antibodies CD19 PE, ZAP-70 FITC (clone 1E7.2) and CD2 APC were used. Leuoperm was used for permeabilization. All samples with over 20% ZAP-70/CD19 double positive B lymphocytes were considered positive. RESULTS: ZAP-70 was absent in a group of 22 patients with B-CLL diagnosed over 18 months from the beginning of this study, while 2 out of 10 (20%) patients from the group of newly diagnosed patients were ZAP-70(+). During follow up both ZAP-70(+) cases showed worsening of their clinical status resulting to death in one patient. In borderline cases with ZAP-70(+) ranging from 10.9-19.9% there was no correlation of the level of ZAP-70 expression with disease activity or clinical scoring systems. CONCLUSION: Very high percentage of ZAP-70(+) cells is a sign of poor prognosis in B-CLL. Borderline expression of ZAP-70, although more frequent, could not be successfully assigned into a risk group. |
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Authors:
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G Marjanovic; I Stanojevic; Lj Jankovic-Velickovic; W Hubl; V Marjanovic |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of B.U.ON. : official journal of the Balkan Union of Oncology Volume: 13 ISSN: 1107-0625 ISO Abbreviation: J BUON Publication Date: 2008 Oct-Dec |
Date Detail:
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Created Date: 2009-01-15 Completed Date: 2009-03-19 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 100883428 Medline TA: J BUON Country: Greece |
Other Details:
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Languages: eng Pagination: 543-6 Citation Subset: IM |
Affiliation:
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Clinic of Hematology, Clinical Centre Nis, Serbia. prof.marjanovic@gmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Female Flow Cytometry Humans Leukemia, Lymphocytic, Chronic, B-Cell / blood*, mortality Male Middle Aged Prognosis Prospective Studies ZAP-70 Protein-Tyrosine Kinase / blood* |
| Chemical | |
Reg. No./Substance:
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EC 2.7.10.2/ZAP-70 Protein-Tyrosine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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