Document Detail


Detection of Vibrio vulnificus biotypes 1 and 2 in eels and oysters by PCR amplification.
MedLine Citation:
PMID:  8919800     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with biotype 1 cells was demonstrated. By the methods described, V. vulnificus cells on a medium (colistin-polymyxin B-cellobiose agar) selective for this pathogen were detectable in oysters harvested in January and March, containing no culturable cells (< 67 CFU/g), as well as in oysters harvested in May and June, containing culturable cells. It was possible to complete DNA extraction, PCR, and gel electrophoresis within 10 h by using the protocol described for oysters. V. vulnificus biotype 2 cells were also detected in eel tissues that had been infected with this strain and subsequently preserved in formalin. The protocol used for detection of V. vulnificus cells in eels required less than 5 h to complete. Optimum MgCl2 concentrations for the PCR of V. vulnificus from oysters and eels were different, although the same primer pair was used for both. This is the first report on the detection of cells of V. vulnificus naturally present in shellfish and represents a potentially powerful method for monitoring this important human and eel pathogen.
Authors:
S S Coleman; D M Melanson; E G Biosca; J D Oliver
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  62     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1997-01-02     Completed Date:  1997-01-02     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1378-82     Citation Subset:  IM    
Affiliation:
Department of Biology, University of North Carolina at Charlotte 28223, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Bacteriological Techniques
Base Sequence
DNA Primers / genetics
DNA, Bacterial / genetics,  isolation & purification
Eels / microbiology*
Evaluation Studies as Topic
Humans
Molecular Sequence Data
Ostreidae / microbiology*
Polymerase Chain Reaction / methods*
Vibrio / classification,  genetics*,  isolation & purification*
Virulence / genetics
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Bacterial
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Identification of novel cry-type genes from Bacillus thuringiensis strains on the basis of restricti...
Next Document:  Distribution of bacterial populations in a stratified fjord (Mariager Fjord, Denmark) quantified by ...