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Detection of Ureaplasma urealyticum and Ureaplasma parvum in amniotic fluid: association with pregnancy outcomes.
MedLine Citation:
PMID:  20504068     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Objective. Ureaplasma urealyticum is one of the organisms most frequently isolated from the amniotic fluid of women with adverse pregnancy. The prevalence of U. urealyticum and U. parvum on samples of amniotic fluid from healthy asymptomatic pregnant women, and whether its detection is associated with P-PROM or preterm birth was investigated. Methods. Transabdominal amniotic fluid obtained from 121 asymptomatic women at 16-20 weeks of gestation were tested for the detection of Ureaplasma spp., using a selective culture media. A Multiplex-Polymerase Chain Reaction (PCR) method was used for the identification of U. parvum and U. urealyticum. Pregnancy outcomes were obtained after the completion of all testing. Results. Ureaplasma spp. was not identified by culture, but was identified by Multiplex-PCR in four subjects, two corresponding to U. parvum and two to U. urealyticum. The women positive to ureaplasmas had normal labor, and babies born from infected-ureaplasmas pregnant women had normal weight birth. Preterm birth with intact membranes was documented in four women, all negative to ureaplasmas, but associated with gestational hypertension, lost of liquids and low weight birth. Conclusions. Multiplex-PCR method was more sensitive that culture in detecting ureaplasma organism in amniotic fluid. No association of ureaplasmas with pregnancy outcomes was found.
Authors:
Nadia Rodríguez; Carmen Fernandez; Yarelys Zamora; Denis Berdasquera; José A Rivera
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Publication Detail:
Type:  Journal Article     Date:  2010-05-26
Journal Detail:
Title:  The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians     Volume:  24     ISSN:  1476-4954     ISO Abbreviation:  J. Matern. Fetal. Neonatal. Med.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101136916     Medline TA:  J Matern Fetal Neonatal Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  47-50     Citation Subset:  IM    
Affiliation:
Tropical Medicine Institute "Pedro Kouri", Havana, Cuba.
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