Document Detail


Detection of TiO(2) Nanoparticles in Cells by Flow Cytometry.
MedLine Citation:
PMID:  22791459     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Evaluation of the potential hazard of man-made nanomaterials has been hampered by a limited ability to observe and measure nanoparticles in cells. A FACSCalibur™ flow cytometer was used to measure changes in light scatter from cells after incubation with TiO(2) nanoparticle. Both the side scatter and forward scatter changed substantially in response to the TiO(2). Between 0.1 and 30 μg/mL TiO(2), the side scatter increased sequentially while the forward scatter decreased, presumably due to substantial light reflection by the TiO(2) particles. At the lowest concentrations of TiO(2) (0.1-0.3 μg/mL), the flow cytometer apparently could detect as few as 5-10 nanoparticles per cell as shown using dark field microscopy. The influence of nanoparticles on the cell cycle was detected by nonionic detergent lysis of nanoparticle-incubated cells. Viability of nanoparticle-treated cells was determined by PI exclusion.These data suggest that the uptake of nanoparticles within cells can be monitored using flow cytometry and confirmed by dark field microscopy. This approach may help fill a critical need to assess the relationship between nanoparticle dose and cellular toxicity. Such experiments could potentially be performed quickly and easily using the flow cytometer to measure both nanoparticle uptake and cellular health.
Authors:
Robert Martin Zucker; Kaitlin Marie Daniel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  906     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-07-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  497-509     Citation Subset:  IM    
Affiliation:
Toxicology Assessment Division, Office of Research and Development, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, NC, USA, zucker.robert@epa.gov.
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