Document Detail


Detection of Salmonella in food samples by the combination of immunomagnetic separation and PCR assay.
MedLine Citation:
PMID:  11334305     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A combination of immunomagnetic separation and polymerase chain reaction (IMS-PCR) was used to detect Salmonella in food samples. Pre-enrichment of samples was combined with filtration through a membrane for the removal of food debris. The IMS-PCR assay combines selective extraction of bacteria by specific antibodies with primer specific PCR amplification that enables to detect Salmonella in non-fatty food samples in 24 h. In comparison with conventional cultural methods, the IMS-PCR is a rapid and specific method. Combined with filtration bags, it partially reduces the negative effects of the food matrix and allows the quick detection of Salmonella cells. The shortened protocols for Salmonella spp. detection described here can improve considerably current methodologies.
Authors:
G Jeníková; J Pazlarová; K Demnerová
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International microbiology : the official journal of the Spanish Society for Microbiology     Volume:  3     ISSN:  1139-6709     ISO Abbreviation:  Int. Microbiol.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-05-03     Completed Date:  2001-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9816585     Medline TA:  Int Microbiol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  225-9     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic. jenikovg@vscht.cz
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MeSH Terms
Descriptor/Qualifier:
Animals
Cheese / microbiology
Colony Count, Microbial
Eggs / microbiology
Filtration / methods
Food Microbiology*
Immunomagnetic Separation / methods*
Meat / microbiology
Polymerase Chain Reaction / methods*
Salmonella enteritidis / genetics,  isolation & purification*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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