Document Detail


Detection of Salmonella enterica serovar Typhi (S. Typhi) by selective amplification of invA, viaB, fliC-d and prt genes by polymerase chain reaction in mutiplex format.
MedLine Citation:
PMID:  16441380     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: Development of a PCR assay that can target multiple genes for rapid detection of Salmonella enterica serovar Typhi (S. Typhi) from water and food samples. METHODS AND RESULTS: PCR primers for invasion, O, H and Vi antigen genes, invA, prt, fliC-d and viaB were designed and used for the rapid detection of S. Typhi by multiplex PCR. Internal amplification control, which co-amplified with prt primers, was also included in the assay. The results showed that all cultures of Salmonella were accurately identified by the assay with no nonspecific amplification in other cultures. The assay had 100% detection probability when a cell suspension of 10(4) CFU ml(-1) (500 CFU per reaction) was used. Salmonella Typhi bacteria were artificially inoculated in the water and food (milk and meat rinse) samples and detected by mPCR after overnight pre-enrichment in buffered peptone water. No Salmonella bacteria could be detected from water samples collected from the field by mPCR or standard culture method. CONCLUSIONS: The developed mPCR assay provides specific detection of S. Typhi. SIGNIFICANCE AND IMPACT OF THE STUDY: Rapid methods for detection of S. Typhi from complex environmental matrices are almost nonexistent. The mPCR assay reported in this study can be useful to identify S. Typhi bacteria in field environmental samples.
Authors:
S Kumar; K Balakrishna; H V Batra
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Letters in applied microbiology     Volume:  42     ISSN:  0266-8254     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-30     Completed Date:  2006-05-25     Revised Date:  2006-09-28    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  149-54     Citation Subset:  IM    
Affiliation:
Division of Microbiology, Defence R and D Establishment, Gwalior, Madhya Pradesh, India. subodh_kumar@email.com
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MeSH Terms
Descriptor/Qualifier:
Antigens, Bacterial / genetics*
Bacterial Proteins / genetics*
Bacterial Typing Techniques
DNA, Bacterial / analysis
Flagellin / genetics*
Polymerase Chain Reaction
Salmonella typhi / genetics,  immunology,  isolation & purification*
Chemical
Reg. No./Substance:
0/Antigens, Bacterial; 0/Bacterial Proteins; 0/DNA, Bacterial; 12777-81-0/Flagellin; 147652-43-5/invA protein, Bacteria

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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