| Detection of SEB gene by bilayer lipid membranes nucleic acid biosensor supported by modified patch-clamp pipette electrode. | |
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MedLine Citation:
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PMID: 17092700 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This work reports a kind of novel bilayer lipid membranes (BLMs) nucleic acid biosensor supported by modified patch-clamp pipette electrode was developed to detect staphylococcus enterotoxins B (SEB) gene. BLMs were formed within 15 min and able to be operated at least 24 h. Hydrophobic dodecane tail (C12) modified 18 bp single-stranded DNA (ssDNA) probe was immobilized on BLMs. The electrochemical currents versus the different concentration of ssDNA probe immobilized on BLMs indicated linearly correlation. The BLMs nucleic acid biosensor was fabricated by selecting the ssDNA probe as the signal sensing element with the concentration of 273.65 ng/mL. The electrochemical performance of the biosensor for the detection of SEB was investigated. The result showed that linear relationship was found between the current and ln(concentration) from 20 to 5000 ng/mL and the detection limit was 20 ng/mL. In addition, the biosensor was specific response to SEB gene and showed no significant current alteration in electrolyte which containing no SEB gene. Finally, Atom Force Microscope (AFM) images could be observed and used to evaluate the superficial microstructure of BLMs, ssDNA immobilized on BLMs and BLMs after hybridization. The BLMs nucleic acid biosensor supported by modified patch-clamp pipette electrode will become a highly sensitive, rapid, selective analytical tool for detection of Staphylococcus aureus, which produce SEB. |
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Authors:
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Nan Liu; Zhixian Gao; HuanYing Zhou; Mingxiang Yue |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-11-07 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 22 ISSN: 0956-5663 ISO Abbreviation: Biosens Bioelectron Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-03-12 Completed Date: 2007-05-25 Revised Date: 2009-07-14 |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
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Languages: eng Pagination: 2371-6 Citation Subset: IM |
Affiliation:
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Institute of Hygiene and Environmental Medicine, Academy of Military Medical Science, Tianjin 300050, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biosensing Techniques* DNA, Bacterial / analysis*, genetics Enterotoxins / genetics* Lipid Bilayers* Patch-Clamp Techniques / instrumentation* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/Enterotoxins; 0/Lipid Bilayers; 39424-53-8/enterotoxin B, staphylococcal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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