Document Detail


Detection of Rickettsia prowazekii in body lice and their feces by using monoclonal antibodies.
MedLine Citation:
PMID:  12202579     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to identify Rickettsia prowazekii in lice, we developed a panel of 29 representative monoclonal antibodies selected from 187 positive hybridomas made by fusing splenocytes of immunized mice with SP2/0-Ag14 myeloma cells. Immunoblotting revealed that 15 monoclonal antibodies reacted with the lipopolysaccharide-like (LPS-L) antigen and 14 reacted with the epitopes of a 120-kDa protein. Only typhus group rickettsiae reacted with the monoclonal antibodies against LPS-L. R. felis, a recently identified rickettsial species, did not react with these monoclonal antibodies, confirming that it is not antigenically related to the typhus group. Monoclonal antibodies against the 120-kDa protein were highly specific for R. prowazekii. We successfully applied a selected monoclonal antibody against the 120-kDa protein to detect by immunofluorescence assay R. prowazekii in smears from 56 wild and laboratory lice, as well as in 10 samples of louse feces infected or not infected with the organism. We have developed a simple, practical, and specific diagnostic assay for clinical specimens and large-scale epidemiological surveys with a sensitivity of 91%. These monoclonal antibodies could be added to the rickettsial diagnostic panel and be used to differentiate R. prowazekii from other rickettsial species.
Authors:
Rong Fang; Linda Houhamdi; Didier Raoult
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  40     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-08-30     Completed Date:  2002-11-14     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3358-63     Citation Subset:  IM    
Affiliation:
Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculty of Medicine, 13385 Marseille, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Bacterial / biosynthesis,  diagnostic use*,  immunology
Antibodies, Monoclonal / biosynthesis,  diagnostic use*,  immunology
Antibody Specificity*
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Feces / microbiology*
Female
Mice
Mice, Inbred BALB C
Pediculus / microbiology*
Rickettsia prowazekii / immunology,  isolation & purification*
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antibodies, Monoclonal
Comments/Corrections

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